Catechol-O-Methyltransferase (COMT) (AA 26-141) antibody

Antigen: Catechol-O-Methyltransferase (COMT)

Synonyms: Comt, D16Wsu103e, D330014B15Rik, COMT, mb-comt, s-comt, MGC140449, comt, MGC154444

Binding Site: AA 26-141

Clonality: Monoclonal (4)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968704

Additional Information

Alternative name: COMT

Immunogen: Mouse COMT

Cross-Reactivity: Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 4

Description: Catechol-O-methyltransferase (COMT) and monoamine oxidase (MAO) are the major mammalian enzymes involved in the degradation of the catecholamine neurotransmitters, dopamine, norepinephrine, and epinephrine. COMT is a Mg2+-dependent enzyme that catalyzes the transfer of methyl groups from S-adenosyl methionine to a hydroxyl group of a catecholic substrate. Two forms of COMT are found in rat brain, a 24 kDa soluble COMT (S-COMT) and a 28 kDa membrane-bound COMT (MB-COMT). COMT is widely expressed in brain, but its importance in catecholamine neurotransmitter degradation relative to MAO varies in different brain regions. In addition, COMT may function primarily in extraneuronal areas, such as in glial cells and postsynaptic neurons. COMT-deficient mice have sex- and region-specific alterations in dopamine levels in the brain, and display impaired emotional reactivity and aggressive behavior. Thus, COMT-mediated degradation of catecholamines in the brain may have important roles in maintaining normal catecholamine levels, as well as normal social behavior. This antibody is routinely tested by western blot analysis.
Synonyms: Catechol-O-Methyltransferase

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 24/28 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: Signaling, Metabolism

Restrictions: For Research Use only

Images

Western blot analysis of COMT on a rat pituitary lysate. Lane 1: 1:10,000, lane 2: 1:20,000, lane 3: 1:40,000 dilution of the anti- COMT antibody.

Immunofluorescence staining of rat cortical neurons.

Publications

Product: Tilgmann, Melen, Lundström et al.: "Expression of recombinant soluble and membrane-bound catechol O-methyltransferase in eukaryotic cells and identification of the respective enzymes in rat brain." in: European journal of biochemistry / FEBS, Vol. 207, Issue 2, pp. 813-21, 1992 (PubMed).

Gogos, Morgan, Luine et al.: "Catechol-O-methyltransferase-deficient mice exhibit sexually dimorphic changes in catecholamine levels and behavior." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 17, pp. 9991-6, 1998 (PubMed).

Werner, Di Rocco, Prikhojan et al.: "COMT-dependent protection of dopaminergic neurons by methionine, dimethionine and S-adenosylmethionine (SAM) against L-dopa toxicity in vitro." in: Brain research, Vol. 893, Issue 1-2, pp. 278-81, 2001 (PubMed).