Hexokinase 2 (HK2) antibody

Details for Product No. ABIN969196
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Antigen
Synonyms HK2, HKII, HXK2, hexokinase-2, AI642394, fi09d05, fi17h06, wu:fi09d05, wu:fi17h06, wu:fi31b04, zgc:55926, ARABIDOPSIS THALIANA HEXOKINASE 2, ATHXK2, F6F22.11, F6F22_11, HEXOKINASE 2, hexokinase 2
Reactivity
Human
(92), (26), (22), (14), (13), (13), (1), (1), (1), (1)
Host
Mouse
(68), (29)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)
(83), (49), (37), (17), (10), (10), (6), (5), (3), (1), (1)
Pubmed 3 references available
Quantity 100 µL
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Catalog No. ABIN969196
363.00 $
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Immunogen Purified recombinant fragment of human HK2 expressed in E. coli.
Clone 3D3
Isotype IgG1
Purification Ascites
Alternative Name HK2
Background Synonyms:
HKII, HXK2, DKFZp686M1669, HK2
The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose mηbolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic AA. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skelηl muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
Molecular Weight 102 kDa
Gene ID 3099
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Hexokinase 2 (HK2) antibody Figure 3: Flow cytometric analysis of K562 cells using HK2 mouse mAb (green) and negative control (purple) .
anti-Hexokinase 2 (HK2) antibody (2) Figure 2: Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues (left) and human lung cancer (right) using HK2 mouse mAb with DAB staining.
anti-Hexokinase 2 (HK2) antibody (3) Figure 1: Western blot analysis using HK2 mouse mAb against Jurkat (1), Hela (2) and HEK293 (3) cell lysate.
Product cited in: Lim, Hao, Shaw et al.: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, 2006 (PubMed).

Paudyal, Oriuchi, Paudyal et al.: "Expression of glucose transporters and hexokinase II in cholangiocellular carcinoma compared using [18F]-2-fluro-2-deoxy-D-glucose positron emission tomography." in: Cancer science, Vol. 99, Issue 2, pp. 260-6, 2008 (PubMed).

He, Bi, Zheng et al.: "Real-time quantitative RT-PCR assessment of PIM-1 and hK2 mRNA expression in benign prostate hyperplasia and prostate cancer." in: Medical oncology (Northwood, London, England), Vol. 26, Issue 3, pp. 303-8, 2009 (PubMed).

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