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MLH1 antibody (MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli)) (AA 381-483)

Details for Product anti-MLH1 Antibody No. ABIN969285, Supplier: Login to see
Antigen
  • CG11482
  • Dmel\\CG11482
  • dmlh-1
  • dmlh1
  • zgc:66301
  • MLH1
  • LOC100232198
  • 1110035C23Rik
  • AI317206
  • AI325952
  • AI561766
  • COCA2
  • FCC2
  • HNPCC
  • HNPCC2
  • hMLH1
Alternatives
anti-Human MLH1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 381-483
20
17
16
13
7
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey
146
40
39
6
5
2
1
1
1
1
1
1
Host
Mouse
96
52
Clonality (Clone)
Monoclonal ()
Conjugate
This MLH1 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
106
40
32
21
21
20
13
11
10
6
3
2
1
1
1
1
Supplier
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Immunogen Purified recombinant fragment of MLH1 (AA 381-483) expressed in E. coli.
Clone 4C9C7
Isotype IgG1
Purification Ascites
Alternative Name MLH1 (MLH1 Antibody Abstract)
Background DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a common characteristic of several different types of human cancers, either due to an MMR gene mutation or promoter-methylation gene silencing. MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integral part of the protein complex responsible for mismatch repair expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. MLH1 also plays a role in meiotic recombination.
Molecular Weight 85 kDa
Gene ID 4292
HGNC 4292
Research Area Cancer, DNA/RNA
Pathways DNA Damage Repair
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Immunocytochemistry (ICC) image for anti-MLH1 antibody (MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli)) (AA 381-483) (ABIN969285) Figure 1: Confocal immunofluorescence analysis of Hela cells using MLH1 mouse mAb (gr...
Immunohistochemistry (IHC) image for anti-MLH1 antibody (MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli)) (AA 381-483) (ABIN969285) Figure 2: Immunohistochemical analysis of paraffin-embedded human rectum cancer (left...
Western Blotting (WB) image for anti-MLH1 antibody (MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli)) (AA 381-483) (ABIN969285) Figure 3: Western blot analysis using MLH1 mouse mAb against Hela (1), MCF-7 (2) and ...
Product cited in: Geary, Sasieni, Houlston et al.: "Gene-related cancer spectrum in families with hereditary non-polyposis colorectal cancer (HNPCC)." in: Familial cancer, Vol. 7, Issue 2, pp. 163-72, 2008 (PubMed).

Latta, Mohan, Issekutz: "CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis." in: Immunology, Vol. 121, Issue 4, pp. 555-64, 2007 (PubMed).

Zeng, Kinsella: "A novel role for DNA mismatch repair and the autophagic processing of chemotherapy drugs in human tumor cells." in: Autophagy, Vol. 3, Issue 4, pp. 368-70, 2007 (PubMed).