MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) (AA 381-483) antibody

Details for Product No. ABIN969285
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Antigen
Synonyms CG11482, Dmel\\CG11482, dmlh-1, dmlh1, zgc:66301, MLH1, LOC100232198, 1110035C23Rik, AI317206, AI325952, AI561766, COCA2, FCC2, HNPCC, HNPCC2, hMLH1
Epitope
AA 381-483
(17), (8), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Monkey
(99), (39), (35), (14), (13), (6), (5), (1), (1), (1), (1), (1)
Host
Mouse
(76), (27)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
(71), (30), (28), (17), (11), (10), (10), (8), (8), (5), (5), (2), (2)
Pubmed 2 references available
Quantity 100 μL
Options
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Catalog No. ABIN969285
363.00 $
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Immunogen Purified recombinant fragment of MLH1 (AA 381-483) expressed in E. coli.
Clone 4C9C7
Isotype IgG1
Purification Ascites
Alternative Name MLH1
Background Synonyms:
FCC2, COCA2, HNPCC
DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a common characteristic of several different types of human cancers, either due to an MMR gene mutation or promoter-methylation gene silencing. MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integral part of the protein complex responsible for mismatch repair expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. MLH1 also plays a role in meiotic recombination.
Molecular Weight 85 kDa
Gene ID 4292
Research Area Cancer, DNA/RNA
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) (AA 381-483) antibody Figure 3: Confocal immunofluorescence analysis of Hela cells using MLH1 mouse mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
anti-MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) (AA 381-483) antibody (2) Figure 2: Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 mouse mAb.
anti-MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli) (MLH1) (AA 381-483) antibody (3) Figure 1: Western blot analysis using MLH1 mouse mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.
Product cited in: Latta, Mohan, Issekutz: "CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis." in: Immunology, Vol. 121, Issue 4, pp. 555-64, 2007 (PubMed).

Geary, Sasieni, Houlston et al.: "Gene-related cancer spectrum in families with hereditary non-polyposis colorectal cancer (HNPCC)." in: Familial cancer, Vol. 7, Issue 2, pp. 163-72, 2008 (PubMed).

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