Nuclear Factor-KB P65 (NFkBP65) antibody

Details for Product No. ABIN969311
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Antigen
Synonyms c-Rel, C-Rel, Xrel2, c-rel, rel-A, v-rel, xrel, NFKB3, p65, NFkB, zgc:100833
Reactivity
Human, Mouse (Murine)
(846), (441), (352), (76), (75), (74), (38), (9), (8), (6), (2), (2), (1), (1)
Host
Mouse
(800), (58), (6), (5), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(22), (19), (15), (11), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(651), (335), (329), (170), (127), (125), (100), (36), (21), (18), (12), (12), (7), (5), (2), (1)
Pubmed 2 references available
Quantity 100 µL
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Catalog No. ABIN969311
363.00 $
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Immunogen Purified recombinant fragment of human NF-kappaB p65 expressed in E. coli.
Clone 6H7
Isotype IgG1
Purification Ascites
Alternative Name NF-kappa B p65
Background Synonyms:
NFκB p65, p65, NFKB3, RELA
Transcription factors of the nuclear factor κ B (NF-κB) /Rel family is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. There are five family members in mammals: RelA (p65), c-Rel, RelB, NF-κB1 (p105/p50) and NF-κB2 (p100/p52). The most studied NF-κB complex consists of the p50 and p65 subunits, both containing a 300 AA region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for the interaction of NF-κB with its inhibitor, IκB. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IκB. This complex prevents nuclear translocation and activity of NF-κB. In response to stimuli such as cytokines, LPS, and viral infections, IκB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IκB/NF-κB complex, allowing the free heterodimeric NF-κB to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the κB site within promoters and enhancers and functions as a transcriptional activator.
Molecular Weight 65 kDa
Gene ID 5970
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Nuclear Factor-KB P65 (NFkBP65) antibody Figure 1: Western blot analysis using NF-κB p65 mouse mAb against Jurkat (1), K562 (2) and NIH/3T3 (3) cell lysate.
Product cited in: DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Yamit-Hezi, Nir, Wolstein et al.: "Interaction of TAFII105 with selected p65/RelA dimers is associated with activation of subset of NF-kappa B genes." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18180-7, 2000 (PubMed).

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