Human TNFRSF10C ELISA Kit

Recommended TNFRSF10C Product (supplied by: Log in to see )

Antigen
Tumor Necrosis Factor Receptor Superfamily, Member 10c, Decoy Without An Intracellular Domain (TNFRSF10C) ELISA Kits
  • CD263
  • DCR1
  • DCR1-TNFR
  • LIT
  • TRAIL-R3
  • TRAILR3
  • TRID
  • TNFRSF10C
  • tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain
  • TNFRSF10C
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
7
1
1
1
Method Type
Sandwich ELISA
Detection Range
15-4000 pg/mL
Minimum Detection Limit
15 pg/mL
Application
ELISA
Options
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Catalog No. ABIN625360
$ 476.30
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN4993231 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 16.4-4000 pg/mL 16.4 pg/mL Log in to see
1 ABIN1446124 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Samples 147 pg/mL Log in to see
1 ABIN5659339 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 16.4 pg/mL - 4000 pg/mL 16.4 pg/mL Log in to see
1 ABIN2685701 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Plasma, Serum 312.5-10000 pg/mL 312.5 pg/mL Log in to see
1 ABIN5510573 Sandwich ELISA Log in to see
1 ABIN2539814 Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 10-800 pg/mL 10 pg/mL Log in to see
1 ABIN1164380 Log in to see

General

Antigen Tumor Necrosis Factor Receptor Superfamily, Member 10c, Decoy Without An Intracellular Domain (TNFRSF10C) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(7), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 15-4000 pg/mL
Minimum Detection Limit 15 pg/mL
Application ELISA
Supplier Log in to see

Product Details TNFRSF10C ELISA Kit

Target details Application Details Handling Images
Purpose Human TRAIL R3 (TNFRSF10C) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensitivity 15 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details TNFRSF10C ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name TRAIL R3 (TNFRSF10C ELISA Kit Abstract)
Background The Human TRAIL R3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TRAIL R3 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human TRAIL R3 coated on a 96-well plate. Standards and samples are pipetted into the wells and TRAIL R3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TRAIL R3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TRAIL R3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
Gene ID 8794
UniProt O14798
Pathways Apoptosis

Application Details

Product Details TNFRSF10C ELISA Kit Target details Handling Images back to top
Application Notes Recommended Dilution for serum and plasma samples3 - 50 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25°C) before use. 2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. Assay Diluent C (Item L) should be used for dilution of culture supernatants and urine. Suggested dilution for normal serum/plasma: 3-50 fold*. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator. 3. Assay Diluent B should be diluted 5-fold with deionized or distilled water before use. 4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µl Assay Diluent A (for serum/plasma samples) or Assay Diluent C (for cell culture supernates/urine) into Item C vial to prepare a 50 ng/ml standard. Dissolve the powder thoroughly by a gentle mix. Add 40 µl TRAIL R3 standard (50 ng/ml) from the vial of Item C, into a tube with 460 µl Assay Diluent A or Assay Diluent C to prepare a 4,000 pg/ml standard solution. Pipette 300 µl Assay Diluent A or Assay Diluent C into each tube. Use the 4,000 pg/ml standard solution to produce a dilution series (shown below). Mix each tube thoroughly before the next transfer. Gently vortex to mix. Assay Diluent A or Assay Diluent C serves as the zero standard (0 pg/ml). 5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer. 6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µl of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4°C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure. 7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 20,000-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 2 µl of HRP-Streptavidin concentrate into a tube with 198 µl 1x Assay Diluent B to prepare a 100-fold diluted HRP- Streptavidin solution (do not store the diluted solution for next day use). Mix through and then pipette 50 µl of prepared 100-fold diluted solution into a tube with 10 ml 1x Assay Diluent B to prepare a final 20,000 fold diluted HRP-Streptavidin solution.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25°C) before use. It is recommended that all standards and samples be run at least in duplicate. 2. Add 100 µl of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4°C with gentle shaking. 3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 µl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. 4. Add 100 µl of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking. 5. Discard the solution. Repeat the wash as in step 3. 6. Add 100 µl of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking. 7. Discard the solution. Repeat the wash as in step 3. 8. Add 100 µl of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking. 9. Add 50 µl of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Restrictions For Research Use only

Handling

Product Details TNFRSF10C ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

Images

Product Details TNFRSF10C ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Tumor Necrosis Factor Receptor Superfamily, Member 10c, Decoy Without An Intracellular Domain (TNFRSF10C) ELISA Kit (ABIN625360) Tumor Necrosis Factor Receptor Superfamily, Member 10c, Decoy Without An Intracellular Domain (TNFRSF10C) ELISA Kit