anti-Human SIRT1 antibody for Immunoprecipitation

Recommended SIRT1 Antibody (supplied by: Log in to see )

Antigen
Sirtuin 1 (SIRT1) Antibodies
  • sirtuin 1
  • AA673258
  • SIR2L1
  • Sir2
  • Sir2a
  • Sir2alpha
  • sir2l1
  • sirtuin
  • SIR2
  • sirtuin 1
  • SIRT1
  • SRT1
  • LOC100349271
  • Sirt1
  • sirt1
Epitope
AA 581-630
29
24
21
18
16
15
15
14
10
6
6
5
4
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
305
139
71
21
14
11
8
7
7
7
5
4
4
3
2
2
2
1
Host
Mouse
207
85
26
6
3
Clonality
Monoclonal
Conjugate
This SIRT1 antibody is un-conjugated
17
14
10
7
7
6
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
254
130
88
65
60
50
41
40
35
7
6
5
4
2
2
2
2
1
1
Options
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Catalog No. ABIN2668278
$ 495.83
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN372326 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN3198318 ChIP IC IF IHC IP WB Rabbit Log in to see Polyclonal
1 ABIN2470013 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN239843 IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN256550 IP WB Rabbit IgG Log in to see Poly6343
1 ABIN5542831 IP WB Mouse IgG2b Log in to see 2G10-F4-D9
1 ABIN151572 IP PLA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1874784 ChIP IF IHC IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1874783 IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN1176381 ICC IHC IP WB Rabbit IgG AA 244-498 Log in to see Polyclonal
1 ABIN2682598 IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2693024 IP WB Rabbit IgG AA 193-747 Log in to see Polyclonal
1 ABIN1824781 IP IHC WB Rabbit IgG AA 702-714 Log in to see Polyclonal
1 ABIN1739281 IHC (p) IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN481177 IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN333854 IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2403466 ChIP IF IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2360975 IC FACS IP IHC WB Rabbit IgG N-Term Log in to see 6k369
1 ABIN214826 IP ELISA WB Mouse IgG1 AA 551-599 Log in to see
1 ABIN2360976 IP IHC WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-SIRT1 antibody for Immunoprecipitation

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Application / Reactivity Human
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ELISA 112 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 41 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 48 Antibodies
Immunofluorescence (IF) 65 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 83 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 59 Antibodies
Immunoprecipitation (IP) 35 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Simple Western (SimWes) 4 Antibodies
Western Blotting (WB) 234 Antibodies

General

Antigen Sirtuin 1 (SIRT1) Antibodies
Epitope AA 581-630
(29), (24), (21), (18), (16), (15), (15), (14), (10), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(305), (139), (71), (21), (14), (11), (8), (7), (7), (7), (5), (4), (4), (3), (2), (2), (2), (1)
Host Mouse
(207), (85), (26), (6), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SIRT1 antibody is un-conjugated
(17), (14), (10), (7), (7), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(254), (130), (88), (65), (60), (50), (41), (40), (35), (7), (6), (5), (4), (2), (2), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-SIRT1 Antibody

Target Details SIRT1 Application Details Handling ProductDetails: References for anti-SIRT1 antibody (ABIN2668278) Images
Purification Protein G Chromatography
Immunogen This SIRT1 antibody was raised against a synthetic peptide corresponding to amino acids 581-630 of human SIRT1.
Clone 2G1-F7
Isotype IgG1

Target Details SIRT1

Product Details anti-SIRT1 Antibody Application Details Handling ProductDetails: References for anti-SIRT1 antibody (ABIN2668278) Images back to top
Antigen
Alternative Name SIRT1 (SIRT1 Antibody Abstract)
Background SIRT1 (Sirtuin 1, SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16. SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans.
Molecular Weight 120 kDa
Gene ID 23411
Research Area Chromatin and Nuclear Signaling, Apoptosis/Necrosis, Proteases
Pathways MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-SIRT1 Antibody Target Details SIRT1 Handling ProductDetails: References for anti-SIRT1 antibody (ABIN2668278) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details ProductDetails: References for anti-SIRT1 antibody (ABIN2668278) Images back to top
Concentration 1 μg/μL
Buffer 10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30 % glycerol, 0.035 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage -20 °C
Storage Comment Antibodies in solution can be stored at -20 °C for 2 years.
Expiry Date 6 months

ProductDetails: References for anti-SIRT1 antibody (ABIN2668278)

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling Images back to top
Product cited in:

Rajendran, Kidane, Yu, Dashwood, Bisson, Löhr, Ho, Williams, Dashwood: "HDAC turnover, CtIP acetylation and dysregulated DNA damage signaling in colon cancer cells treated with sulforaphane and related dietary isothiocyanates." in: Epigenetics, Vol. 8, Issue 6, pp. 612-23, 2013

Vaquero, Scher, Lee, Erdjument-Bromage, Tempst, Reinberg: "Human SirT1 interacts with histone H1 and promotes formation of facultative heterochromatin." in: Molecular cell, Vol. 16, Issue 1, pp. 93-105, 2004

Images

Product Details anti-SIRT1 Antibody Target Details SIRT1 Application Details Handling ProductDetails: References for anti-SIRT1 antibody (ABIN2668278) back to top
Supplier Images
Western Blotting (WB) image for anti-SIRT1 antibody (Sirtuin 1) (AA 581-630) (ABIN2668278) SIRT1 mAb (Clone 2G1/F7) tested by Western blot. Nuclear extract (20 µg) of CMSL0 cel...