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Fas (TNF Receptor Superfamily, Member 6) (FAS) (Soluble) Protein

Details for Product No. ABIN967460
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Protein Name
Synonyms CD95, APT1, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6, lpr, APO1, TNFR6, Tnfrsf6, AI196731, FAS
Protein Characteristics
Soluble
Origin
Human
(27), (7), (5), (1), (1), (1), (1), (1), (1)
Host
Please enquire
(16), (8), (5), (4), (3)
Protein Type Recombinant
Application
Western Blotting (WB), Standard (STD)
Pubmed 3 references available
Quantity 10 µg
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Catalog No. ABIN967460
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Licensed for Research Purposes Only. Commercial use requires license from Boyce Thompson Institute for Plant Research.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purity Purified
Alternative Name Fas
Background Fas (APO-1, CD95) is an ~42 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung. FasL is a 40 kDa TNF family member membrane protein that induces apoptosis by binding to Fas, its cell-surface receptor. FasL is expressed on activated T and NK cells. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development. Recombinant soluble human Fas was expressed in the Baculovirus Expression Vector System and purified from insect cell lysate using an anti-Fas antibody. This form of Fas lacks the transmembrane domain of the Fas receptor, and has been referred to as FasDeltaTM. It has a 63-base pair deletion starting at nucleotide sequence (700) GA TCC AGA and ending at nucleotide sequence GTT TGG G (762). The purity and the integrity of the protein were verified by SDS-PAGE, and ELISA. FasDeltaTM has a reduced molecular weight of ~40 kDa.
Application Notes Purified FasDeltaTM can be used as a positive control for western blot analysis of Fas. 1 µg of protein can easily be visualized on a Commasie-stained gel as a band of ~40 kDa. For western blot analysis, 1 µg or less is sufficient depending on the detection method (less is needed for enhanced chemiluminescence than for standard alkaline phosphatase detection). Purified FasDeltaTM can also be used as a standard in Fas ELISA assays. The recombinant FasDeltaTM protein should not be used in functional assays, as it does not appear to assume a biologically active conformation.
Restrictions For Research Use only
Format Liquid
Buffer Aqueous buffered solution containing BSA and glycerol.
Storage -20 °C
General Cheng, Zhou, Liu et al.: "Protection from Fas-mediated apoptosis by a soluble form of the Fas molecule." in: Science (New York, N.Y.), Vol. 263, Issue 5154, pp. 1759-62, 1994 (PubMed).

Takahashi, Tanaka, Brannan et al.: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, 1994 (PubMed).

Tanaka, Suda, Takahashi et al.: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, 1995 (PubMed).

Hosts (16), (8), (5), (4), (3)
Reactivities (27), (7), (5), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967460
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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