FAS Protein (AA 26-173) (His tag)

Catalog No. ABIN7274587

Product Details

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Protein Type: Recombinant

Protein Characteristics: AA 26-173

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FAS protein is labelled with His tag.

Purpose: Human Fas/TNFRSF6/CD95 Protein

Sequence: Gln26-Asn173

Characteristics: Recombinant Human Fas/TNFRSF6/CD95 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln26-Asn173.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: Fas (FAS Products)

Synonyms: ALPS1A Protein, APO-1 Protein, APT1 Protein, CD95 Protein, FAS1 Protein, FASTM Protein, TNFRSF6 Protein, AI196731 Protein, APO1 Protein, TNFR6 Protein, Tnfrsf6 Protein, lpr Protein, Fas cell surface death receptor Protein, Fas (TNF receptor superfamily member 6) Protein, FAS Protein, Fas Protein, fas Protein

Background: CD95 (also known as Fas) is a member of the tumor necrosis factor receptor (TNFR) superfamily. Its cognate ligand, CD95L, is implicated in immune homeostasis and immune surveillance. Mutations in this receptor are associated with a loss of apoptotic signaling and have been detected in an autoimmune disorder called autoimmune lymphoproliferative syndrome (ALPS) type Ia, which shares some clinical features with systemic lupus erythematosus (SLE).

Molecular Weight: 13.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity