DARPP32 antibody (N-Term)

Catalog No. ABIN372605

There are 8+ publications for this product available. The Rabbit Polyclonal anti-DARPP32 antibody is suitable to detect DARPP32 in samples from Human, Mouse, Rat, Cow, Chicken, Dog, Xenopus laevis and Zebrafish (Danio rerio). It has been validated for WB, IF and IHC (fro).

Quick Overview for DARPP32 antibody (N-Term) (ABIN372605)

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Dog, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DARPP32 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DARPP32 Antibody

Binding Specificity: N-Term

Specificity: Specific for the ~32k DARPP-32 protein.

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, Xenopus and Zebrafish.
Species reactivity (tested):Rat.

Purification: Affinity Chromatography.

Immunogen: Peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000. Immunohistochemistry: 1/250.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-DARPP32 antibody (ABIN372605)

Bonito-Oliva, DuPont, Madjid, Ögren, Fisone: "Involvement of the Striatal Medium Spiny Neurons of the Direct Pathway in the Motor Stimulant Effects of Phencyclidine." in: The international journal of neuropsychopharmacology, (2016) (PubMed).

Gulati, Cheung, Antrobus, Church, Harding, Tung, Rimmington, Ma, Ron, Lehner, Ashcroft, Cox, Coll, ORahilly, Yeo: "Role for the obesity-related FTO gene in the cellular sensing of amino acids." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 7, pp. 2557-62, (2013) (PubMed).

Rewal, Jurd, Gill, He, Ron, Janak: "Alpha4-containing GABAA receptors in the nucleus accumbens mediate moderate intake of alcohol." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 2, pp. 543-9, (2009) (PubMed).

Haycock, Becker, Ang, Furukawa, Hornykiewicz, Kish: "Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum." in: Journal of neurochemistry, Vol. 87, Issue 3, pp. 574-85, (2003) (PubMed).

Waymire, Haycock: "Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells." in: Journal of neurochemistry, Vol. 81, Issue 3, pp. 589-93, (2002) (PubMed).

Kish, Kalasinsky, Derkach, Schmunk, Guttman, Ang, Adams, Furukawa, Haycock: "Striatal dopaminergic and serotonergic markers in human heroin users." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 24, Issue 5, pp. 561-7, (2001) (PubMed).

Zhu, Klimek, Haycock, Ordway: "Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain." in: Journal of neuroscience methods, Vol. 99, Issue 1-2, pp. 37-44, (2000) (PubMed).

Zhu, Klimek, Dilley, Haycock, Stockmeier, Overholser, Meltzer, Ordway: "Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression." in: Biological psychiatry, Vol. 46, Issue 9, pp. 1275-86, (1999) (PubMed).

Target Details for DARPP32

Target: DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))

Alternative Name: DARPP32

Background: DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002).Synonyms: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B

Gene ID: 84152

NCBI Accession: NP_001229393

UniProt: Q9UD71