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RYK Receptor-Like Tyrosine Kinase (RYK) ELISA Kits
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Method Type
Sandwich ELISA
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Catalog No. ABIN4886279
$ 728.20
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN4884386 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell and Tissue Lysate Log in to see
1 ABIN1164624 Log in to see

Similar RYK ELISA Kits

Application / Reactivity Human


Antigen RYK Receptor-Like Tyrosine Kinase (RYK) ELISA Kits
Epitope pTyr
Reactivity Human
Kits with alternative reactivity to:
(2), (1)
Method Type Sandwich ELISA
Application ELISA
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Product Details RYK ELISA Kit

Target details Application Details Handling Images
Purpose Custom Human Phosphotyrosine RYK ELISA Kit. This assay semi-quantitatively measures phosphotyrosine RYK in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
Detection Method Colorimetric
Analytical Method Semi-Quantitative
Specificity The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-RYK.
  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Biotinylated Anti-Phosphotyrosine Antibody
  • Stop Solution
  • Assay Diluent(s)
  • Positive Control Sample
  • Lysis Buffer
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Target details

Product Details RYK ELISA Kit Application Details Handling Images back to top
Alternative Name RYK (RYK ELISA Kit Abstract)
Gene ID 6259
UniProt P34925
Research Area Kinases/Phosphatases, Wnt, Stem Cells, Tyrosine Kinases
Pathways RTK Signaling, WNT Signaling, Regulation of Cell Size

Application Details

Product Details RYK ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Plate Pre-coated
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only


Product Details RYK ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months