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RYK ELISA Kit

RYK Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4884386
  • Target See all RYK ELISA Kits
    RYK (RYK Receptor-Like Tyrosine Kinase (RYK))
    Reactivity
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human Ryk ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes human Ryk. Other species not yet determined.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product RYK ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all RYK ELISA Kits
    RYK (RYK Receptor-Like Tyrosine Kinase (RYK))
    Alternative Name
    Ryk (RYK Products)
    Synonyms
    RYK ELISA Kit, jtk5 ELISA Kit, ryk1 ELISA Kit, jtk5a ELISA Kit, d3s3195 ELISA Kit, wu:fb37b12 ELISA Kit, wu:fi05b06 ELISA Kit, zgc:158381 ELISA Kit, MGC114645 ELISA Kit, D3S3195 ELISA Kit, JTK5 ELISA Kit, JTK5A ELISA Kit, RYK1 ELISA Kit, AW536699 ELISA Kit, ERK-3 ELISA Kit, Vik ELISA Kit, receptor-like tyrosine kinase ELISA Kit, receptor-like tyrosine kinase S homeolog ELISA Kit, tyrosine-protein kinase RYK ELISA Kit, RYK ELISA Kit, ryk ELISA Kit, ryk.S ELISA Kit, LOC100541106 ELISA Kit, Ryk ELISA Kit
    Gene ID
    6259
    UniProt
    P34925
    Pathways
    RTK Signaling, WNT Signaling, Regulation of Cell Size
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