Cholesterol 25-Hydroxylase (CH25H) ELISA Kits

Human Cholesterol 25-Hydroxylase (CH25H) interaction partners

1. We examined whether 25HC restricts infection by mammalian reovirus. Results suggest that 25HC inhibits the efficiency of cellular entry of reovirus virions, which may require specific endosomal membrane dynamics for efficient membrane penetration.

2. We detected Cholesterol 25-hydroxylase, the enzyme responsible for 25-HC production, in human spermatozoa at the level of the neck and the post acrosomal area. Upon incubation with spermatozoa, 25-HC induced calcium and cholesterol transients in connection with the acrosomal reaction.

3. Exome analysis in 212 multiple sclerosis and 14 neuromyelitis optica patients identified a rare CH25H p.Q17H mutation in two NMO patients of Asian ancestry. In addition, association analysis of common CH25H variants.

4. Infection with HCV causes up-regulation of interferon-inducible CH25H in vivo.

5. These results demonstrate that CH25H constitutes a primary innate response against hepatitis C virus infection through regulating host lipid metabolism.

6. Cholesterol 25-hydroxylase production by dendritic cells and macrophages is regulated by type I interferons.

7. Based on the results of genome-wide screens, along with biological studies, we selected three genes as candidates for AD risk factors: ATP-binding cassette transporter A1 (ABCA1), cholesterol 25-hydroxylase (CH25H) and cholesterol 24-hydroxylase (CH24H).

Mouse (Murine) Cholesterol 25-Hydroxylase (CH25H) interaction partners

1. results indicate that osteoarthritis is a disease associated with metabolic disorders and suggest that targeting the CH25H-CYP7B1-RORalpha axis of cholesterol metabolism may provide a therapeutic avenue for treating osteoarthritis.

2. Adenovirus-mediated Ch25h overexpression in mice improved glucose tolerance and insulin sensitivity and lowered HOMA-IR. Our data suggest that low hepatic Ch25h levels could be considered a risk marker for unhealthy obesity.

3. Ch25h-deficient mice exhibit increased sensitivity to septic shock, exacerbated experimental autoimmune encephalomyelitis, and a stronger ability to repress bacterial growth.

4. Disruption of Roralpha1 and cholesterol 25-hydroxylase expression attenuates phagocytosis in male Roralphasg/sg mice.

5. Findings indicate that CYP7B1 and HSD3B7, as well as CH25H, have essential roles in controlling oxysterol production in lymphoid tissues.

6. We found that expression of Ch25h is upregulated following L. monocytogenes infection

7. Data show that The increased Ch25h expression was dependent on Toll-like receptor 4 signaling.

8. deletion of the mouse cholesterol 25-hydroxylase gene caused an increase in serum IgA.

Pig (Porcine) Cholesterol 25-Hydroxylase (CH25H) interaction partners

1. CH25H and 25HC inhibited the replication of porcine transmissible gastroenteritis virus (TGEV). Taken together, CH25H as a natural host restriction factor could inhibit porcine epidemic diarrhea virus and TGEV infection.

2. Collectively, these findings demonstrate that CH25H is an intrinsic host restriction factor in pseudorabies virus infection of porcine alveolar macrophages.