anti-Cytochrome B5 Type A (Microsomal) (CYB5A) Antibodies

Human Cytochrome B5 Type A (Microsomal) (CYB5A) interaction partners

1. A homodimer model can resolve the conundrum as to how cytochrome P450 oxidoreductase and cytochrome b5 compete for the same binding site on cytochrome P450c17. (Review)

2. Data suggest CYP17A1, with electron donor NADPH-P450 reductase, is inherently distributive enzyme but that some processivity is present; some 17alpha-hydroxy pregnenolone formed does not dissociate from CYP17A1 before conversion to dehydroepiandrosterone; CYB5A does not enhance reaction by decreasing k(off) of ligands of CYP17A1. (CYP17A1 = cytochrome P450, family 17, subfamily A, polypeptide 1; CYB5A = cytochrome b5)

3. Data suggest that cytochrome b5 (CYB5) and cytochrome b5 reductase 3 (CYB5R3) can reduce human cytoglobin (CYGB) and zebrafish cytoglobins at rates up to 250-fold higher than those reported for the known physiological substrates, hemoglobin and myoglobin; the three proteins (CYB5+CYB5R3+CYGB) appear to constitute a metabolon involved in generation of nitric oxide.

4. Thus, although Mn-b5 binds to CYP17A1, it is unable to enhance the lyase reaction, strongly suggesting that cyt b5 has a redox effector role in enhancement of the CYP17A1 mediated lyase reaction necessary for androgen synthesis.

5. This study constructed a model of the membrane-bound full-length human P450 1A2-cyt b5 complex. The model was assembled from several parts using a multiscale modeling approach covering all-atom and coarse-grained molecular dynamics (MD).

6. We conclude that cytochrome b5 can influence the electronic conductivity of cytochrome P450c17 via allosteric, protein-protein interactions.

7. Variants in CYB5A and CYB5R3 should be considered in the evaluation of breast cancer risk in African American women.

8. CYB5A is the first RA susceptibility gene involved in androgen synthesis. Our functional analysis of SNP rs1790834 indicates that it contributes to the sex bias observed in RA.

9. both area and ratio of 3betaHSD and CYB5A double positive cells, which could represent the hybrid cells of zona fasciculate and zona reticularis, are correlated with adrenal development and could influence age-related serum androstenedione levels.

10. Two acidic residues, Glu-48 and Glu-49, of cytochrome b5 are essential for stimulating the 17,20-lyase activity of CYP17A1.

11. raises the possibility that inhibition of CYB5A-deregulated downstream pathways, such as those involving TRAF6, may favor autophagy-mediated cancer cell death in selected subgroups of patients

12. CYB5A, which has a role in stearyl-CoA-desaturase activity, and RNF10, with an unknown role in weight regulating pathways, associated with adiposity and nominally increased the risk for T2D in American Indians.

13. Cytochrome b5 seems to play the key role in the formation of lipid-radical cycles in membranes.

14. Results define CYB5A as a novel prognostic factor for PDAC that exerts its tumor-suppressor function through autophagy induction and TRAF6 modulation.

15. CYP17A1/b5 interaction is stronger when the hydroxylase substrate pregnenolone is present in the CYP17A1 active site than when the lyase substrate 17alpha-hydroxypregnenolone is in the active site.

16. Identification of the first human CYB5A missense mutation as the cause of isolated 17,20 lyase deficiency in three individuals with 46,XY disorder of sex development.

17. Human adrenal cells that express both 3beta-hydroxysteroid dehydrogenase type 2 (HSD3B2) and cytochrome b5 (CYB5A) contribute to adrenal androstenedione production

18. Cygb has a nitric-oxide dioxygenase function and ascorbate and cytochrome b(5) have roles as reductants

19. We demonstrated 17,20-lyase deficiency due to an aberrant CytB5.

20. novel cSNPs associated with significantly altered protein expression and/or hydroxylamine reduction activities, but only minimally impact overall observed phenotypic variability

Cow (Bovine) Cytochrome B5 Type A (Microsomal) (CYB5A) interaction partners

1. the conserved Tyr30 in Cyt b5 is not replaceable due to the presence of both the H-bond network and hydrophobic interaction in the secondary sphere of the heme active site.

2. The study computes electron transfer rates for the [myoglobin(wt), cytochrome b5] complex.

3. The solution structure of the oxidized cytochrome b(5) mutant V61H.

4. the effects on the heme microenvironment of cytochrome b(5) caused by the mutation from Val45 to Tyr45, His45 and Glu45

5. Results from three combined experiments demonstrate that the conserved negatively charged region of cytb5 surrounding the solvent-exposed heme edge is involved in the binding interaction with horse heart cytochrome c.

6. From a series of site-directed mutations on Cyt b5 this report assesses the contributions of amino acid residues that are expected to influence the docking and electron transfer (ET) between Cyt b5 and horse heart Cyt c.

7. simulations provided qualitative microscopic explanations of many of the differences in physical properties between outer mitochondrial membrane CYB5 isoform and microsomal CYB5 and two mutants in terms of localized changes in structure and flexibility

Pig (Porcine) Cytochrome B5 Type A (Microsomal) (CYB5A) interaction partners

1. Searching the porcine CYB5 gene for mutations, examine its expression, and identify genetic polymorphism in terms of androstenone levels in testis.

2. the c.-8G>T SNP in the CYB5A gene has a significant but modest effect on boar taint in male pigs and could be useful in some breeds as part of a panel of SNP markers in a marker-assisted selection programme to produce low boar taint pigs

3. These results demonstrate the differential effects of two forms of CYB5 on the three activities of porcine CYP17A1 and show that CYB5B does not stimulate the andien-beta synthase activity of CYP17A1.

Horse (Equine) Cytochrome B5 Type A (Microsomal) (CYB5A) interaction partners

1. It was concluded that CYP3A related metabolism in horse is not solely dependent on the expression of the enzyme but also on adequate levels of NADPH P450 reductase and cytochrome b(5).

Mouse (Murine) Cytochrome B5 Type A (Microsomal) (CYB5A) interaction partners

1. Demonstration of the physiologic importance of cytochrome b5 activating the 17,20-lyase reaction and the production of 19-carbon sex steroids from 21-carbon precursors in mice.

2. Cytochrome b5 and cytokeratin 17 are biomarkers in bronchoalveolar fluid signifying onset of acute lung injury.

Rabbit Cytochrome B5 Type A (Microsomal) (CYB5A) interaction partners

1. Data indicate not only that binding of b5 and P450 reductase on the proximal surface of P450 2B4 results in electron transfer but also that each redox partner transmits unique structural information to the active site proton delivery network.

2. the observation of weak and nonspecific interactions between CYP2B4 and cytb5 in micelles suggests that lipid bilayer structures and low curvature membrane surface are preferable for CYP2B4-cytb5 complex formation.

3. Data suggest that the cytb5-cytP4502B4 complex structure will help unravel the mechanism by which cytb5 regulates catalysis by cytP4502B4.

4. Two different types of cytochrome b5 orientations are present within a MFO system: the first allowing potential cytochrome b5 electron donation to P450, the second one inducing cytochrome b5 modulation of P450 structural changes.