anti-Cytochrome B5 Type A (Microsomal) (CYB5A) Antibodies

Human Cytochrome B5 Type A (Microsomal) (CYB5A) interaction partners

1. A homodimer model can resolve the conundrum as to how cytochrome P450 (show CYP Antibodies) oxidoreductase (show TXNRD1 Antibodies) and cytochrome b5 compete for the same binding site on cytochrome P450c17 (show CYP17A1 Antibodies). (Review)

2. Data suggest CYP17A1 (show CYP17A1 Antibodies), with electron donor NADPH-P450 reductase (show POR Antibodies), is inherently distributive enzyme but that some processivity is present; some 17alpha-hydroxy pregnenolone formed does not dissociate from CYP17A1 (show CYP17A1 Antibodies) before conversion to dehydroepiandrosterone; CYB5A does not enhance reaction by decreasing k(off) of ligands of CYP17A1 (show CYP17A1 Antibodies). (CYP17A1 (show CYP17A1 Antibodies) = cytochrome P450 (show CYP Antibodies), family 17, subfamily A (show CYP Antibodies), polypeptide 1 (show CYP Antibodies); CYB5A = cytochrome b5)

3. Data suggest that cytochrome b5 (CYB5) and cytochrome b5 reductase 3 (CYB5R3 (show CYB5R3 Antibodies)) can reduce human cytoglobin (CYGB (show CYGB Antibodies)) and zebrafish cytoglobins at rates up to 250-fold higher than those reported for the known physiological substrates, hemoglobin (show HBB Antibodies) and myoglobin (show MB Antibodies); the three proteins (CYB5+CYB5R3 (show CYB5R3 Antibodies)+CYGB (show CYGB Antibodies)) appear to constitute a metabolon involved in generation of nitric oxide.

4. Thus, although Mn-b5 binds to CYP17A1 (show CYP17A1 Antibodies), it is unable to enhance the lyase reaction, strongly suggesting that cyt b5 (show CYT-B5 Antibodies) has a redox effector role in enhancement of the CYP17A1 (show CYP17A1 Antibodies) mediated lyase reaction necessary for androgen synthesis.

5. This study constructed a model of the membrane-bound full-length human P450 (show CYP2B6 Antibodies) 1A2-cyt b5 (show CYT-B5 Antibodies) complex. The model was assembled from several parts using a multiscale modeling approach covering all-atom and coarse-grained molecular dynamics (MD).

6. We conclude that cytochrome b5 can influence the electronic conductivity of cytochrome P450c17 (show CYP17A1 Antibodies) via allosteric, protein-protein interactions.

7. Variants in CYB5A and CYB5R3 (show CYB5R3 Antibodies) should be considered in the evaluation of breast cancer risk in African American women.

8. CYB5A is the first RA susceptibility gene involved in androgen synthesis. Our functional analysis of SNP rs1790834 indicates that it contributes to the sex bias observed in RA.

9. both area and ratio of 3betaHSD (show HSD3B1 Antibodies) and CYB5A double positive cells, which could represent the hybrid cells of zona fasciculate and zona reticularis, are correlated with adrenal development and could influence age-related serum androstenedione levels.

10. Two acidic residues, Glu (show DCTN1 Antibodies)-48 and Glu (show DCTN1 Antibodies)-49, of cytochrome b5 are essential for stimulating the 17,20-lyase activity of CYP17A1 (show CYP17A1 Antibodies).

Cow (Bovine) Cytochrome B5 Type A (Microsomal) (CYB5A) interaction partners

1. the conserved Tyr30 in Cyt b5 (show CYT-B5 Antibodies) is not replaceable due to the presence of both the H-bond network and hydrophobic interaction in the secondary sphere of the heme active site.

2. The study computes electron transfer rates for the [myoglobin (show MB Antibodies)(wt), cytochrome b5] complex.

3. The solution structure of the oxidized cytochrome b(5) mutant V61H.

4. the effects on the heme microenvironment of cytochrome b(5) caused by the mutation from Val45 to Tyr45, His45 and Glu45

5. Results from three combined experiments demonstrate that the conserved negatively charged region of cytb5 (show CYT-B5 Antibodies) surrounding the solvent-exposed heme edge is involved in the binding interaction with horse heart cytochrome c (show CYCS Antibodies).

6. From a series of site-directed mutations on Cyt b5 (show CYT-B5 Antibodies) this report assesses the contributions of amino acid residues that are expected to influence the docking and electron transfer (ET) between Cyt b5 (show CYT-B5 Antibodies) and horse heart Cyt c (show CYCS Antibodies).

7. simulations provided qualitative microscopic explanations of many of the differences in physical properties between outer mitochondrial membrane CYB5 isoform and microsomal CYB5 and two mutants in terms of localized changes in structure and flexibility

Pig (Porcine) Cytochrome B5 Type A (Microsomal) (CYB5A) interaction partners

1. Searching the porcine CYB5 gene for mutations, examine its expression, and identify genetic polymorphism in terms of androstenone levels in testis.

2. the c.-8G>T SNP in the CYB5A gene has a significant but modest effect on boar taint in male pigs and could be useful in some breeds as part of a panel of SNP markers in a marker-assisted selection programme to produce low boar taint pigs

3. These results demonstrate the differential effects of two forms of CYB5 on the three activities of porcine CYP17A1 (show CYP17A1 Antibodies) and show that CYB5B (show CYB5B Antibodies) does not stimulate the andien-beta synthase activity of CYP17A1 (show CYP17A1 Antibodies).

Horse (Equine) Cytochrome B5 Type A (Microsomal) (CYB5A) interaction partners

1. It was concluded that CYP3A related metabolism in horse is not solely dependent on the expression of the enzyme but also on adequate levels of NADPH P450 reductase (show POR Antibodies) and cytochrome b(5).

Mouse (Murine) Cytochrome B5 Type A (Microsomal) (CYB5A) interaction partners

1. Demonstration of the physiologic importance of cytochrome b5 activating the 17,20-lyase reaction and the production of 19-carbon sex steroids from 21-carbon precursors in mice.

2. Cytochrome b5 and cytokeratin 17 (show KRT17 Antibodies) are biomarkers in bronchoalveolar fluid signifying onset of acute lung injury.

Rabbit Cytochrome B5 Type A (Microsomal) (CYB5A) interaction partners

1. Data indicate not only that binding of b5 and P450 reductase (show POR Antibodies) on the proximal surface of P450 (show POR Antibodies) 2B4 (show CD244 Antibodies) results in electron transfer but also that each redox partner transmits unique structural information to the active site proton delivery network.

2. the observation of weak and nonspecific interactions between CYP2B4 and cytb5 (show CYT-B5 Antibodies) in micelles suggests that lipid bilayer structures and low curvature membrane surface are preferable for CYP2B4-cytb5 (show CYT-B5 Antibodies) complex formation.

3. Data suggest that the cytb5 (show CYT-B5 Antibodies)-cytP4502B4 complex structure will help unravel the mechanism by which cytb5 (show CYT-B5 Antibodies) regulates catalysis by cytP4502B4.

4. Two different types of cytochrome b5 orientations are present within a MFO system: the first allowing potential cytochrome b5 electron donation to P450 (show POR Antibodies), the second one inducing cytochrome b5 modulation of P450 (show POR Antibodies) structural changes.