anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) Antibodies

PPIA encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. Additionally we are shipping PPIA Kits (77) and PPIA Proteins (57) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
PPIA 5478 P62937
PPIA 25518 P10111
PPIA 268373 P17742
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Top anti-PPIA Antibodies at antibodies-online.com

Showing 10 out of 248 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-PPIA Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:312500  Positive Control:  Human brain 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated IHC (p), WB Western blot analysis of Cyclophilin A using anti- Cyclophilin A antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: rat lung tissue lysates,   Lane 2: rat brain tissue lysates,   Lane 3: mouse lung tissue lysates,  Lane 4: HELA whole Cell lysates.  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Cyclophilin A antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclophilin A at approximately 18KD. The expected band size for Cyclophilin A is at 18KD. IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody . Cyclophilin A was detected in immunocytochemical section of THP-1 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Cyclophilin A Antibody  overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen. 100 μg Log in to see 4 to 6 Days
$280.00
Details
Cow Rabbit Un-conjugated WB 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Goat Un-conjugated ELISA, WB ABIN185542 (2µg/ml) staining of human lymph node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. 100 μg Log in to see 6 to 7 Days
$358.02
Details
Human Rabbit Un-conjugated ICC, IF, IHC (p), WB WB Image Cyclophilin A antibody detects Cyclophilin A protein by western blot analysis. Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody , at a dilution of 1:5000. IHC-P Image Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using Cyclophilin A, antibody at 1:500 dilution. 100 μL Log in to see 1 to 2 Days
$358.60
Details
Cow Rabbit Un-conjugated IHC, WB WB Suggested Anti-PPIA Antibody Titration:  2.5ug/ml  Positive Control:  HepG2 cell lysate PPIA is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells Human Intestine 100 μL Log in to see 2 to 3 Days
$229.00
Details
Human Rabbit Un-conjugated FACS, IHC (p), WB Western blot analysis of PPIA Antibody (N-term) (ABIN389487) in T47D cell line and mouse brain,bladder tissue lysates (35 µg/lane). PPIA (arrow) was detected using the purified polyclonal antibody.(2 µg/mL) Formalin-fixed and paraffin-embedded human brain tissue reacted with PPIA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 400 μL Log in to see 10 to 11 Days
$335.50
Details
Human Rabbit Un-conjugated ELISA, IHC (p), WB Immunohistochemistry (IHC) analysis of paraffin-embedded Rat Brain, antibody was diluted at 1:100. 100 μL Log in to see 16 Days
$181.73
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Immunohistochemistry-Paraffin: Cyclophilin A Antibody [NBP1-30993] - Paraffin-embedded MDA-MB-468 xenograft, using antibody at 1:500 dilution. Western Blot: Cyclophilin A Antibody [NBP1-30993] - Raji whole cell extracts and cytoplasma+membrane and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cyclophilin A antibody at a dilution of 1:5000. 100 μL Log in to see 8 to 11 Days
$514.63
Details
Cow Goat Un-conjugated EIA, WB   0.1 mg Log in to see Available
$462.00
Details

Top referenced anti-PPIA Antibodies

  1. Human Monoclonal PPIA Primary Antibody for ELISA, WB - ABIN562340 : Rho, Roehrl, Wang: Tissue proteomics reveals differential and compartment-specific expression of the homologs transgelin and transgelin-2 in lung adenocarcinoma and its stroma. in Journal of proteome research 2009 (PubMed)
    Show all 3 Pubmed References

  2. Human Polyclonal PPIA Primary Antibody for IF (p), IHC (p) - ABIN759716 : Kang, Kim, Hong, Park, Heinsen, Lee, Ravid, Ferrer, Yoo, Kwon, Park: Large-scale analysis of posttranslational modifications in the hippocampus of patients with Alzheimer's disease using pI shift and label-free quantification without enrichment. in Analytical and bioanalytical chemistry 2014 (PubMed)

  3. Human Polyclonal PPIA Primary Antibody for ELISA, WB - ABIN250906 : Berthoux, Sebastian, Sokolskaja, Luban: Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells. in Proceedings of the National Academy of Sciences of the United States of America 2005 (PubMed)

  4. Human Polyclonal PPIA Primary Antibody for IHC, IHC (p) - ABIN4301644 : Yamamoto, Kudo, Peng, Takata, Takakura, Teduka, Fujii, Mitamura, Taga, Uchida, Naito: Identification of aldolase A as a potential diagnostic biomarker for colorectal cancer based on proteomic analysis using formalin-fixed paraffin-embedded tissue. in Tumour biology 2016 (PubMed)

More Antibodies against PPIA Interaction Partners

Xenopus laevis Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) interaction partners

Human Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) interaction partners

  1. Here, the binding site of AIF (show AIFM1 Antibodies)(Delta1-121) and AIF (show AIFM1 Antibodies)(370-394) on CypA has been mapped by NMR spectroscopy and biochemical studies, and a molecular model of the complex has been proposed. The authors show that AIF (show AIFM1 Antibodies)(370-394) interacts with CypA on the same surface recognized by AIF (show AIFM1 Antibodies)(Delta1-121) protein and that the region is very close to the CypA catalytic pocket.

  2. CyPA was significantly increased in convalescent COPD (show ARCN1 Antibodies) patients and further elevated in COPD (show ARCN1 Antibodies) patients with acute exacerbation.

  3. CypA expression was not an independent prognostic factor, but correlated with poor prognosis of lung adenocarcinoma

  4. Extracellular cyclophilin A augments platelet-dependent thrombosis and thromboinflammation.

  5. the increased expression of CyPA in ectopic endometrial tissue is associated with endometrioma recurrences and vascularity.

  6. Taken together, these findings reveal an essential role of CypA in boosting RIG-I (show DDX58 Antibodies)-mediated antiviral immune responses by controlling the ubiquitination of RIG-I (show DDX58 Antibodies) and MAVS (show MAVS Antibodies).

  7. Data show that cyclophilin A (CypA) is up-regulated in oral squamous cell carcinoma (OSCC) arising from oral submucous fibrosis (OSF (show OSTF1 Antibodies)).

  8. K308 of NS5A is indispensable for both RNA and NS5B binding, whereas W316 is essential for protein-protein interactions with CypA and NS5B.

  9. PPIA is differentially expressed in lesional biopsy specimens from patients with atopic eczema relative to normal skin

  10. inosine-5'-monophosphate dehydrogenase 2 (IMPDH2 (show IMPDH2 Antibodies)) is an intracellular target of the PPIA-Sanglifehrin A binary complex.

Mouse (Murine) Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) interaction partners

  1. Extracellular cyclophilin A augments platelet-dependent thrombosis and thromboinflammation.

  2. Endothelial cell-derived CypA (especially AcK (show TNK2 Antibodies)-CypA) causes pulmonary artery hypertension by a presumptive mechanism involving increased EC apoptosis, inflammation, and oxidative stress.

  3. ox-LDL-induced CyPA secretion requires vesicle transportation, actin remodeling and ROCK-dependent diphosphorylation of myosin light chains in vascular smooth muscle cells.

  4. knockdown of CD147 on Hepa1-6 cells resulted in significantly increased T cells chemotaxis induced by CypA both in vivo and in vitro.

  5. CypA dually exerts pro-osteogenic and anti-osteoclastic effects.

  6. Extracellular CyPA activates platelets via cluster of differentiation 147-mediated phosphoinositid-3-kinase/Akt (show AKT1 Antibodies)-signaling, leading to enhanced adhesion and thrombus formation independently of intracellular CyPA.

  7. CypA provided stability for NF-kappaB (show NFKB1 Antibodies) p65 (show NFkBP65 Antibodies) and promoted NF-kappaB (show NFKB1 Antibodies) p65 (show NFkBP65 Antibodies) nuclear translocation, resulting in increased nuclear accumulation and enhanced NF-kappaB (show NFKB1 Antibodies) activity.

  8. Newborn CypA-deficient pups exhibited generalized, pronounced skeletal defects, while high-resolution micro-computed tomography analyses of the femurs and lumbar vertebrae revealed delayed or incomplete endochondral ossification.

  9. Cyclophilin A secretion was increased in lymphocytes from atherogenic mice.

  10. CD137 (show TNFRSF9 Antibodies)-CD137L (show TNFSF9 Antibodies) interactions mediated via regulation of CyPA contribute to the progression of atherosclerosis.

PPIA Antigen Profile

Protein Summary

This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.

Gene names and symbols associated with PPIA

  • peptidylprolyl isomerase A (PPIA) antibody
  • peptidylprolyl isomerase Aa (cyclophilin A) (ppiaa) antibody
  • peptidylprolyl isomerase A (cyclophilin A) L homeolog (ppia.L) antibody
  • cyclophilin A (Cypa) antibody
  • cyclophilin A (CNB01230) antibody
  • cyclophilin A (CNB01290) antibody
  • cyclophilin A (Tc00.1047053506925.300) antibody
  • cyclophilin A (Tb11.03.0250) antibody
  • cyclophilin a (CYPA) antibody
  • peptidylprolyl isomerase A (cyclophilin A) (Ppia) antibody
  • peptidylprolyl isomerase A (cyclophilin A) (ppia) antibody
  • peptidylprolyl isomerase A (Ppia) antibody
  • peptidylprolyl isomerase A (cyclophilin A) (PPIA) antibody
  • 2700098C05 antibody
  • Cphn antibody
  • CYCA antibody
  • CyP-18 antibody
  • CyP-A antibody
  • Cyp1 antibody
  • cypA antibody
  • CYPH antibody
  • fa93g09 antibody
  • MGC75715 antibody
  • MT-ND1 antibody
  • MTND1 antibody
  • NADH1 antibody
  • ND1 antibody
  • ppia antibody
  • PPIA-2 antibody
  • PPIase A antibody
  • wu:fa93g09 antibody
  • wu:fb05e11 antibody
  • wu:fb13h02 antibody
  • wu:fb15a05 antibody

Protein level used designations for PPIA

PPIase A , cyclophilin A , cyclophilin T , cyclophilin T-cell , cyclosporin A-binding protein , peptidyl-prolyl cis-trans isomerase A , rotamase A , 2-peptidylprolyl isomerase A , peptidylprolyl isomerase A (cyclophilin A) , cyclophilin 18 , immunophilin , cyclophilin-like , T cell cyclophilin , cyclophilin , cyclophilin a , p1B15 , p31 , Cyclophilin A , Cyclosporin A-binding protein , Rotamase A , SP18 , NADH dehydrogenase subunit 1 , NADH-ubiquinone oxidoreductase chain 1 , Peptidyl-prolyl cis-trans isomerase A

GENE ID SPECIES
281418 Bos taurus
336612 Danio rerio
100008724 Oryctolagus cuniculus
397637 Sus scrofa
463386 Pan troglodytes
398630 Xenopus laevis
692737 Bombyx mori
574102 Macaca mulatta
5478 Homo sapiens
3255869 Cryptococcus neoformans var. neoformans JEC21
3255972 Cryptococcus neoformans var. neoformans JEC21
3554513 Trypanosoma cruzi strain CL Brener
3664945 Trypanosoma brucei brucei strain 927/4 GUTat10.1
5652501 Leishmania major strain Friedlin
25518 Rattus norvegicus
394470 Xenopus (Silurana) tropicalis
100594480 Nomascus leucogenys
268373 Mus musculus
776282 Gallus gallus
608151 Canis lupus familiaris
100173012 Pongo abelii
493966 Felis catus
443258 Ovis aries
100973085 Pan paniscus
100722609 Cavia porcellus
100769490 Cricetulus griseus
101925040 Macaca fascicularis
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