SPO11 Meiotic Protein Covalently Bound To DSB Homolog (S. Cerevisiae) Proteins (SPO11)

Human SPO11 Meiotic Protein Covalently Bound To DSB Homolog (S. Cerevisiae) (SPO11) interaction partners

1. SPO11 gene C631T polymorphism may contribute as a genetic factor susceptible to cause male infertility in Chinese.

2. Data suggest that the combined genotypes of glutathionine S-transferase GSTM1 (-/-), GSTT1 (+/+), GSTP1 (AA) and meiotic recombination protein SPO11 (CT) may be associated with idiopathic male infertility in ethnic Han Chinese.

3. Polymorphisms within the SPO11 gene are linked to the susceptibility of azoospermia and oligozoospermia male infertility.

4. SPO11 might has an effect on premorbid functioning, which increase susceptibility for idiopathic male reproduction

5. Mutations in the human SPO11 gene are not common causes of infertility in man

6. Data show that there is no association between SPO11 gene mutation and premature ovarian failure.

Mouse (Murine) SPO11 Meiotic Protein Covalently Bound To DSB Homolog (S. Cerevisiae) (SPO11) interaction partners

1. These data suggest that Tex19.1 and Ubr2 (show UBR2 Proteins) are required for mouse spermatocytes to accumulate sufficient Spo11-dependent recombination to ensure that the homology search is consistently successful, and reveal a hitherto unknown genetic pathway promoting meiotic recombination in mammals.

2. If SPO11 expression is reduced and DNA double-strand breaks at zygonema are decreased (approximately 40 % below wild-type level), meiotic chromosome pairing is normal.

3. suppression of genetic recombination in the pseudoautosomal region and at subtelomeres in mice with a hypomorphic Spo11 allele

4. our results indicate that in addition to asynapsis, persistent SPO11-induced DSBs are important for the initiation of MSCI and MSUC, and that SPO11-independent DNA repair foci contribute to the MSUC response in oocytes.

5. Mice with greater or fewer copies of the Spo11 gene--with correspondingly greater or fewer numbers of early recombination foci--exhibited relatively invariant crossover numbers.

6. chromosome synapsis defects and sexually dimorphic meiotic progression in Spo11 deficient mice

7. Spo11 is not required for Ig hypermutation or class switch recombination

8. In Spo11 -/- cells, SMC1 (show SMC1A Proteins) localization on meiotic chromosomes is mainly associated with homologous or non-homologous synapsed regions, whereas SMC3 (show SMC3 Proteins) localized throughout the chromosomes. Both localized along chromosome cores of Spo11 null spermatocytes.

9. meiotic double-stranded breaks are processed by endonucleolytic cleavage that releases Spo11 attached to an oligonucleotide with a free 3'-OH

10. The presence of these L-foci in Spo11(-/-) spermatocytes raises the interesting possibility of yet uncharacterized alterations in DNA or chromosome structure in Spo11(-/-) cells.