anti-Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) Antibodies

Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, PTEN and DAXX. Additionally we are shipping USP7 Proteins (7) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
USP7 252870 Q6A4J8
USP7 7874 Q93009
USP7 360471 Q4VSI4
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Top anti-USP7 Antibodies at antibodies-online.com

Showing 10 out of 232 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated ICC, IF, IHC (p), IP, IHC, PLA, WB Western Blot: USP7 Antibody [ABIN151954] - Detection of Human and Mouse USP7 by Western Blot (h and m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-USP7 antibody ABIN151954 (lot ABIN151954-3) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. USP7 was also immunoprecipitated by a previous lot (lot ABIN151954-2) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B). 100 μL Log in to see 7 to 9 Days
$559.35
Details
Cow Rabbit Un-conjugated WB Host: Rat Target Name: USP7 Sample Tissue: Rat Brain Antibody Dilution: 1ug/ml WB Suggested Anti-USP7 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: A549 cell lysate 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Mouse Un-conjugated FACS, IHC, IHC (p), WB 100 μL Log in to see 11 to 14 Days
$507.83
Details
Human Mouse Un-conjugated IHC, IHC (p), WB 100 μL Log in to see 11 to 14 Days
$507.83
Details
Human Rabbit Un-conjugated EIA, WB USP7 Antibody (Center) western blot analysis in A2058 cell line lysates (35 µg/lane). This demonstrates the USP7 antibody detected the USP7 protein (arrow). 0.4 mL Log in to see 6 to 8 Days
$484.00
Details
Human Rabbit Un-conjugated EIA, IHC (p), WB 0.4 mL Log in to see 6 to 8 Days
$484.00
Details
Chimpanzee Rabbit Un-conjugated EIA, WB   0.1 mg Log in to see 4 to 8 Days
$619.30
Details
Human Rabbit Un-conjugated IF, IHC, WB Immunohistochemistry of paraffin-embedded mouse heart using USP7 antibody at dilution of 1:200 (400x lens). 100 μL Log in to see 16 Days
$366.77
Details
Human Mouse Un-conjugated ELISA, WB Figure 1: Western blot analysis using HAUSP mouse mAb against MCF-7 (1) cell lysate. Dilution: 1/500 - 1/2000 Figure 2: Flow cytometric analysis of A549 cells using anti-TCF3 mAb (green) and negative control (purple). Dilution: 1:200~~400 100 μL Log in to see 8 to 11 Days
$491.79
Details
Human Rabbit Un-conjugated ELISA, IHC, WB Western blot analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: human Hela whole cell lysates,  Lane 2: human 22RV1 whole cell lysates,  Lane 3: human A431 whole cell lysates,  Lane 4: human HepG2 whole cell lysates,  Lane 5: human HepG2 whole cell lysates,  Lane 6: human SGC-7901 whole cell lysates,  Lane 7: human A549 whole cell lysates,  Lane 8: rat brain tissue lysates, Lane 9: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAUSP/USP7 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAUSP/USP7 at approximately 140KD. The expected band size for HAUSP/USP7 is at 128KD. IHC analysis of HAUSP/USP7 using anti-HAUSP/USP7 antibody . HAUSP/USP7 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-HAUSP/USP7 Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg Log in to see 4 to 6 Days
$280.00
Details

Top referenced anti-USP7 Antibodies

  1. Human Polyclonal USP7 Primary Antibody for ICC, IF - ABIN151954 : Canning, Boutell, Parkinson, Everett: A RING finger ubiquitin ligase is protected from autocatalyzed ubiquitination and degradation by binding to ubiquitin-specific protease USP7. in The Journal of biological chemistry 2004 (PubMed)
    Show all 25 Pubmed References

  2. Human Monoclonal USP7 Primary Antibody for ELISA, WB - ABIN966249 : Abély: [Treatment of airway inflammation in cystic fibrosis]. in Archives de pediatrie : organe officiel de la Societe francaise de pediatrie 2007 (PubMed)
    Show all 5 Pubmed References

  3. Human Monoclonal USP7 Primary Antibody for ELISA, WB - ABIN969186 : Becker, Marchenko, Maurice, Moll: Hyperubiquitylation of wild-type p53 contributes to cytoplasmic sequestration in neuroblastoma. in Cell death and differentiation 2007 (PubMed)
    Show all 3 Pubmed References

  4. Human Polyclonal USP7 Primary Antibody for IHC (p), ELISA - ABIN545112 : DAndrea, Pellman: Deubiquitinating enzymes: a new class of biological regulators. in Critical reviews in biochemistry and molecular biology 1999 (PubMed)
    Show all 3 Pubmed References

  5. Human Monoclonal USP7 Primary Antibody for EIA, WB - ABIN1109455 : Lu, Ma, Nguyen, Jones, Oren, Donehower: The Wip1 Phosphatase acts as a gatekeeper in the p53-Mdm2 autoregulatory loop. in Cancer cell 2007 (PubMed)
    Show all 3 Pubmed References

  6. Cow (Bovine) Polyclonal USP7 Primary Antibody for WB - ABIN2787924 : Nie, Zhang, Dai, Zhang, Zhao, Xu, Chen, Wang, Wang, Qiao: Nicotine Induced Murine Spermatozoa Apoptosis via Up-Regulation of Deubiquitinated RIP1 by Trim27 Promoter Hypomethylation. in Biology of reproduction 2016 (PubMed)

  7. Human Monoclonal USP7 Primary Antibody for ELISA, WB - ABIN4880500 : Higginbotham, OShea: Adenovirus E4-ORF3 Targets PIAS3 and Together with E1B-55K Remodels SUMO Interactions in the Nucleus and at Virus Genome Replication Domains. in Journal of virology 2015 (PubMed)

More Antibodies against USP7 Interaction Partners

Fruit Fly (Drosophila melanogaster) Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) interaction partners

  1. reveal a conserved mechanism by which Ci/Gli is stabilized by a deubiquitination enzyme and identify Usp7/HUASP as a critical regulator of Hh signaling and potential therapeutic target for Hh-related cancers

  2. Genetic and gene expression analyses suggested that GMPS/USP7 acts as a transcriptional corepressor

  3. GMP synthetase stimulates histone H2B deubiquitylation by the epigenetic silencer USP7

Mouse (Murine) Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) interaction partners

  1. Evidence shows that USP7 localizes to replication foci independently of DNMT1 and indicates that USP7 does not control cell cycle-dependent levels of DNMT1.

  2. Pit1 deletion inhibited USP7/IRS1 dissociation upon insulin stimulation.

  3. EPOP (E130012A19Rik) interacts with elongin B, elongin C, and USP7 to modulate the chromatin landscape.

  4. USP7 is overexpressed and regulates homologous recombination repair in chronic lymphocytic leukemia cells.

  5. Findings demonstrate a crucial role of HAUSP in regulating N-Myc function in neuroblastoma in vivo and suggest that HAUSP inhibition is a potential therapy for MYCN-amplified tumors.

  6. The present study identified USP7 and TDP-43 as the regulators of CRY1 and CRY2, underscoring the significance of the stability control process of CRY proteins for period determination in the mammalian circadian clockwork.

  7. demonstrate that genotoxic stress stimulates Cry1 phosphorylation and its deubiquitination by Herpes virus associated ubiquitin-specific protease (Hausp, a.k.a Usp7), stabilizing Cry1 and shifting circadian clock time

  8. Our results demonstrate that the SCML2/USP7 complex constitutes a novel molecular pathway in modulating the epigenetic state of sex chromosomes during male meiosis

  9. USP7-mediated mono-deubiquitination of FoxO1 results in suppression of FoxO1 transcriptional activity through decreased FoxO1 occupancy on the promoters of gluconeogenic genes.

  10. Early adipogenesis is regulated through USP7-mediated deubiquitination of the histone acetyltransferase TIP60.

  11. The ubiquitination-deubiquitination cascade mediated by the TRIM27-USP7 complex plays an important role in TNF-alpha-induced apoptosis.

  12. the stability and activity of PPARgamma2 are modulated by the deubiquitinating activity of HAUSP, which may be a target for the development of anti-diabetic drugs.

  13. data reveal a molecular mechanism in which rapid temporal control of Foxp3 expression in Treg cells can be regulated by USP7, thereby modulating Treg cell numbers and function

  14. These results indicate that HAUSP-mediated p53 regulation is crucial for brain development, and also suggest that both the p53-dependent and the p53-independent functions of HAUSP contribute to the neonatal lethality of hausp-mutant mice.

  15. HAUSP knockout mice embryos showed p53 activation, but no increase in apoptosis.

  16. USP7 (HAUSP) is proteolytically processed upon dexamethasone-, gamma-irradiation-, and antigen-induced cell death. Such processing of HAUSP does not occur in caspase 3-/- thymocytes.

  17. mHAUSP functions as a deubiquitinating enzyme for p53

  18. mHAUSP regulates apoptosis of cervical adenocarcinoma cells

Human Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7) interaction partners

  1. Study demonstrated that USP7 expression in osteosarcoma (OS) tumor tissues was markedly higher than that in the paired surrounding tissues, and high USP7 expression was positively correlated with the TNM stage and metastasis in patients with OS.

  2. The deubiquitination activity of USP7 was inhibited by the ubiquitin-conjugating enzyme UbcH6. UVSSA deficient in the interaction with USP7 is ubiquitinated and degraded by the proteasome.

  3. Full kinetic analysis and multiple X-ray crystal structures indicate that electrostatic interactions in the distal ''switching loop'' region and local packing in the hydrophobic core mediate subtle but significant conformational changes that modulate USP7 activation.

  4. show that BRE facilitates deubiquitylation of CDC25A by recruiting ubiquitin-specific-processing protease 7 (USP7) in the presence of DNA damage

  5. High expression of USP7 is frequent in HCC tissues, which promotes tumor proliferation and invasion, and is correlated with a poor overall survival.

  6. REVIEW: USP7 plays crucial roles in diverse array of cellular and biological processes including tumour suppression, cell cycle, DNA repair, chromatin remodelling, and epigenetic regulation.

  7. USP7 depletion in APC-mutated colorectal cancer inhibits Wnt activation by restoring beta-catenin ubiquitination, drives differentiation, and suppresses xenograft tumor growth.

  8. USP7 is RNF169 interacting protein.Expression of USP7 and RNF169 positively correlated in breast cancer.

  9. protein deubiquitinase USP7 is an essential player in osteogenic differentiation of Human adipose-derived stem cells.

  10. ubiquitin-specific protease 7 (USP7) deubiquitinase targeting by vIRF-3 (in addition to previously reported USP7 binding by vIRF-1 and vIRF-4); the importance of vIRF-1 and vIRF-3 interactions with USP7 for latent PEL cell growth and viability; and the positive and negative contributions, respectively, of USP7 targeting by vIRF-1 and vIRF-3 to HHV-8 productive replication.

  11. demonstrate that both USP7 and various USP7 substrates are subjected to Lys48-mediated ubiquitin modification, consistent with increased proteasomal degradation of these proteins because of USP7 inhibition

  12. Here we study the transition between USP7 states. We provide a crystal structure of USP7(CD123) and show that catalytic domain CD and the first 3 Ubl domains Ubl123 are connected via an extended charged alpha helix. Mutational analysis is used to determine whether the charge and rigidity of this 'connector helix' are important for full USP7 activity

  13. the overexpression of USP7 might promote cell proliferation by deubiquitinating Ki-67 protein

  14. these data identify DUB3 and USP7 as factors that regulate DNA replication by controlling Geminin protein stability, and suggest that USP7 may be involved in Geminin dysregulation during breast cancer progression.

  15. Molecular mechanisms of USP7 substrate recognition and C-terminal activation have been described.

  16. USP7 promotes breast carcinogenesis by stabilizing PHF8 and upregulating cyclin A2. and the interaction between USP7 and PHF8 is augmented during DNA damage.

  17. Data suggest that SIRT7 undergoes Lys-63 polyubiquitination, later removed by USP7 to repress enzymatic activity of SIRT7; USP7 and SIRT7 regulate gluconeogenesis via expression of glucose-6-phosphatase catalytic subunit (G6PC); SIRT7 targets G6PC promoter through ELK4. (SIRT7 = sirtuin 7; USP7 = ubiquitin specific peptidase 7; G6PC = glucose-6-phosphatase catalytic subunit; ELK4 = transcription factor ELK4)

  18. USP7 is overexpressed and regulates homologous recombination repair in chronic lymphocytic leukemia cells.

  19. Findings demonstrate a crucial role of HAUSP in regulating N-Myc function in neuroblastoma in vivo and suggest that HAUSP inhibition is a potential therapy for MYCN-amplified tumors.

  20. findings suggest that Usp7 is important for MB cell proliferation and metastasis by activating Shh pathway, and is a putative therapeutic target for MBs

USP7 Antigen Profile

Protein Summary

Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, PTEN and DAXX. Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis. Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity. In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. Involved in cell proliferation during early embryonic development. Involved in transcription-coupled recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6 (By similarity).

Gene names and symbols associated with USP7

  • Ubiquitin-specific protease 7 (Usp7) antibody
  • ubiquitin carboxyl-terminal hydrolase 7 (LOC100201254) antibody
  • ubiquitin specific peptidase 7 (USP7) antibody
  • ubiquitin specific peptidase 7 (Usp7) antibody
  • 2210010O09Rik antibody
  • AA409944 antibody
  • AA617399 antibody
  • AU019296 antibody
  • AW548146 antibody
  • C80752 antibody
  • CG1490 antibody
  • Dmel\\CG1490 antibody
  • HAUSP antibody
  • TEF1 antibody
  • UBP antibody
  • USP7 antibody

Protein level used designations for USP7

CG1490-PB , Usp7-PB , ubiquitin specific peptidase 7 (herpes virus-associated) , ubiquitin carboxyl-terminal hydrolase 7-like , deubiquitinating enzyme 7 , herpesvirus-associated ubiquitin-specific protease , mHAUSP , ubiquitin carboxyl-terminal hydrolase 7 , ubiquitin specific protease 7 , ubiquitin thioesterase 7 , ubiquitin thiolesterase 7 , ubiquitin-specific-processing protease 7 , Herpes virus-associated ubiquitin-specific protease , ubiquitin specific protease 7 (herpes virus-associated) , herpes-virus-associated ubiquitin-specific protease , rHAUSP , ubiquitin-specific peptidase 7

GENE ID SPECIES
32169 Drosophila melanogaster
100201254 Hydra magnipapillata
100468835 Ailuropoda melanoleuca
252870 Mus musculus
7874 Homo sapiens
360471 Rattus norvegicus
395126 Gallus gallus
479854 Canis lupus familiaris
100190899 Sus scrofa
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