WNK Lysine Deficient Protein Kinase 1 Proteins (WNK1)

Human WNK Lysine Deficient Protein Kinase 1 (WNK1) interaction partners

1. Kidney-specific WNK1 isoform is a potent activator of WNK4 and NCC-mediated sodium/chloride transport.

2. The formation of WNK bodies requires an evolutionarily conserved cysteine-rich hydrophobic motif harbored within a unique N-terminal exon of KS-WNK1. It is proposed that WNK bodies are not pathological aggregates, but rather are KS-WNK1-dependent microdomains of the DCT cytosol that modulate WNK signaling during physiological shifts in potassium balance.

3. The identified novel nonsense mutation in WNK1/HSN2 in an Iranian Hereditary sensory and autonomic neuropathy type 2 pedigree presents allelic heterogeneity of this gene in different populations

4. SNP rs1468326 of WNK1, rs6749447 of STK39, and WNK1 haplotype AGACAGGAATCGT were associated with hypertension in Tibetan individuals

5. A new WNK1/HSN2 founder mutation has been found in Japanese patients with hereditary sensory and autonomic neuropathy.

6. Small interfering RNA knockdown of MAPK7 demonstrated that MAPK7 regulates a subset of WNK1-regulated genes and controls the migration and cell proliferation

7. The AA genotype of WNK1 rs1468326 is related with an increased risk for preeclampsia.

8. Mutated WNK1 is a driver of chronic lymphocytic leukemia.

9. Using small-interfering RNA-mediated WNK1 knockdown, we show autophagosome formation and autophagic flux are accelerated. In cells with reduced WNK1, basal and starvation-induced autophagy is increased. We also show that depletion of WNK1 stimulates focal class III phosphatidylinositol 3-kinase complex (PI3KC3) activity, which is required to induce autophagy.

10. WNK1 and the associated phosphorylation of the PCF11 CID act to promote transcript release from chromatin-associated Pol II, which in turn facilitates mRNA export to the cytoplasm

11. Akt3 constitutively suppresses macropinocytosis in macrophages through a novel WNK1/SGK1/Cdc42 pathway.

12. NKCC1 and KCCs are coordinately regulated by L-WNK1 isoforms.

13. Single nucleotide polymorphisms STK39 and WNK were associated with hypertension and BP in our multicenter Belgian case-control study

14. these data provide insight into the mechanism regulating Cl(-) homeostasis in immature neurons, and suggest that WNK1-regulated changes in KCC2 phosphorylation contribute to the developmental excitatory-to-inhibitory GABA sequence.

15. Identify a new chimeric transcript generated by the fusion of WNK1 and B4GALNT3 genes, correlated with B4GALNT3 overexpression in papillary thyroid carcinoma.

16. a novel gene, WNK1, for susceptibility to pelvic organ prolapse

17. findings indicate that the proline-rich exons are modular cassettes that convert WNK1 into a NEDD4-2 substrate, thereby linking aldosterone and other NEDD4-2-suppressing antinatriuretic hormones to NCC phosphorylation status.

18. enhances BK channel function by reducing ERK1/2 signaling-mediated lysosomal degradation of the channel

19. conclude that although multiple candidate genes are involved in development of hypertension, the genetic polymorphism in WNK1 is not a major contributor to the observed variability in blood pressure and familial clustering risk of hypertension

20. study identifies a separation of functions for the WNK1-activated protein kinases OSR1 and SPAK in mediating proliferation, invasion, and gene expression in endothelial cells

Pig (Porcine) WNK Lysine Deficient Protein Kinase 1 (WNK1) interaction partners

1. HSN2 is a nervous system-specific exon of the WNK1 gene (WNK1/HSN2 isoform).

Chimpanzee WNK Lysine Deficient Protein Kinase 1 (WNK1) interaction partners

1. HSN2 is a nervous system-specific exon of the WNK1 gene (WNK1/HSN2 isoform).

Rhesus Monkey WNK Lysine Deficient Protein Kinase 1 (WNK1) interaction partners

1. HSN2 is a nervous system-specific exon of the WNK1 gene (WNK1/HSN2 isoform).

Horse (Equine) WNK Lysine Deficient Protein Kinase 1 (WNK1) interaction partners

1. HSN2 is a nervous system-specific exon of the WNK1 gene (WNK1/HSN2 isoform).

Mouse (Murine) WNK Lysine Deficient Protein Kinase 1 (WNK1) interaction partners

1. These findings suggest that WNK1 is involved in the physiological regulation of mammalian skeletal muscle hypertrophy and atrophy via interactions with FOXO4.

2. Although these results confirm that familial hyperkalemic hypertension caused by WNK1 mutations is dependent on the activation of SPAK, they suggest that WNK1 and WNK4 play different roles in the distal nephron.

3. WNK1 is required for high potassium intake-induced stimulation of ROMK activity in convoluted kidney tubules.

4. Regulation of erythrocyte Na(+)/K(+)/2Cl(-) cotransport by an oxygen-switched kinase cascade.

5. The formation of WNK bodies requires an evolutionarily conserved cysteine-rich hydrophobic motif harbored within a unique N-terminal exon of KS-WNK1. It is proposed that WNK bodies are not pathological aggregates, but rather are KS-WNK1-dependent microdomains of the DCT cytosol that modulate WNK signaling during physiological shifts in potassium balance.

6. GABAAR-dependent regulation of KCC2 diffusion involves phosphorylation of its T906/T1007 residues by WNK1.

7. reduced age-dependent weight gain of WNK1 TG mice seems to be related with the decreased Kir6.2 expression via WNK1- and WNK4-regulated protein stability of Kir6.2.

8. The differential regulation of ROMK, large-conductance Ca(2+)-activated K(+) (BK) channel, BK-alpha and NKCC2 between female and male mice, at least, were partly mediated via WNK1 pathway, which may contribute to the sexual dimorphism of plasma K(+) and blood pressure control.

9. this study identified the WNK1 kinase as a regulator of both integrin-mediated adhesion and T cell migration

10. Akt3 constitutively suppresses macropinocytosis in macrophages through a novel WNK1/SGK1/Cdc42 pathway.

11. Electrophysiological analysis showed that WNK1/HSN2 shifted the concentration of Cl(-)such that GABA signaling resulted in a less hyperpolarized state (increased neuronal activity) rather than a more hyperpolarized state (decreased neuronal activity) in mouse spinal nerves.

12. A high-K(+) diet induced an increase in L-WNK1 expression selectively in intercalated cells and may contribute to enhanced BK channel expression and K(+) secretion in collecting ducts.

13. these data provide insight into the mechanism regulating Cl(-) homeostasis in immature neurons, and suggest that WNK1-regulated changes in KCC2 phosphorylation contribute to the developmental excitatory-to-inhibitory GABA sequence.

14. findings indicate that the proline-rich exons are modular cassettes that convert WNK1 into a NEDD4-2 substrate, thereby linking aldosterone and other NEDD4-2-suppressing antinatriuretic hormones to NCC phosphorylation status.

15. enhances BK channel function by reducing ERK1/2 signaling-mediated lysosomal degradation of the channel

16. increased protein expression levels of WNK1 and WNK4 kinases cause PHAII by KLHL3 R528H mutation due to impaired KLHL3-Cullin3-mediated ubiquitination.

17. study identifies a separation of functions for the WNK1-activated protein kinases OSR1 and SPAK in mediating proliferation, invasion, and gene expression in endothelial cells

18. WNK1 stimulates the activity of the Na-Cl cotransporter via SPAK, an effect antagonized by WNK4.

19. Results show that the expression profiles of Wnk1 isoforms change during the development, and that the expression of the Wnk1 splice variant containing the Hsn2 exon is prominent during developing and in adult mouse tissues.

20. subset of integral membrane proteins, therefore, requires an early dislocation event to expose part of their luminal domain to the cytosol, before HRD1-mediated polyubiquitination and dislocation