Rabbit anti-Human IgA (Heavy Chain) Antibody (Texas Red (TR)) - Preadsorbed

Details for Product No. ABIN100727
Antigen
Epitope
Heavy Chain
69
49
23
11
10
6
6
1
1
Reactivity
Human
126
60
38
28
13
11
10
10
10
10
6
5
4
4
3
3
3
3
2
2
1
1
1
1
Host
Rabbit
143
87
57
13
7
7
2
Clonality
Polyclonal
Conjugate
Texas Red (TR)
37
34
32
21
20
14
6
5
4
4
4
4
4
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Options
Immunogen

Immunogen: Human IgA alpha heavy chain

Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Labeling Ratio 2.8
Antigen
Background

Synonyms: Rabbit Anti Human IgA (alpha chain) Antibody Texas Red™ Conjugated, Rabbit Anti-Human IgA (alpha chain) Antibody Texas Red™ Conjugated, Rabbit Anti Human IgA Antibody Texas Red™ Conjugated

Background: Anti-Human IgA Texas Red Antibody generated in rabbit detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Notes

Application Note: Anti-Human IgA Texas Red Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Comment

Texas Red™ is a registered trademark of Molecular Probes Inc.

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Product is photosensitive and should be protected from light.
Storage RT,4 °C,-20 °C
Expiry Date 12 months