Goat anti-Mouse IgA, IgG, IgM Antibody (Biotin)
-
- Target See all IgA, IgG, IgM products
- IgA, IgG, IgM
-
Reactivity
- Mouse
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- Biotin
-
Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 610-106-130
- Supplier
- Rockland
- Purpose
- Mouse IgG IgA IgM (H&L) Antibody Biotin Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. This reagent is suitable for the detection of all mouse immunoglobulin subclasses, isotypes and chain combinations.
- Characteristics
- Anti-Mouse IgG Biotin Antibody generated in goat detects reactivity to Mouse IgG.
- Purification
- This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Immunogen
- Optional[Immunogen]: Mouse IgG IgA and IgM whole molecule
- Isotype
- IgG
-
-
- Application Notes
- Application Note: Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:500,000 Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- Concentration
- 2.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
-
- Target
- IgA, IgG, IgM
- Alternative Name
- IgG,IgA,IgM (IgA, IgG, IgM Products)
- Target Type
- Antibody
- Background
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
-