Rabbit anti-Cow (Bovine) IgG (Heavy & Light Chain) Antibody - Preadsorbed

Details for Product No. ABIN100899
Antigen
Epitope
Heavy & Light Chain
1569
134
100
56
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Cow (Bovine)
355
342
336
233
232
122
113
87
80
72
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Rabbit
846
798
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Bovine IgG whole molecule

Isotype IgG
Specificity IgG (H&L)
Characteristics Anti-Bovine IgG whole molecule antibody generated in goat detects specifically human IgG whole molecule. This secondary antibody anti-Bovine is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Bovine IgG (H&L) antibody is ideal for investigators in Immunology, Cancer, and Micrbology research.
Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Sterility Sterile filtered
Antigen
Background

Synonyms: rabbit anti-Bovine IgG Antibody, Rb-a-Bovine IgG, Bovine IgG Antibody in Rabbit, Human Secondary Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Anti-Bovine IgG (H&L) antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.

Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Bovine IgG (H&L) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions For Research Use only
Format Liquid
Concentration 2.0 mg/mL
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Expiry Date 12 months