Rabbit anti-Cow (Bovine) IgG (Fc Region) Antibody - Preadsorbed

Details for Product No. ABIN100938
Antigen
Epitope
Fc Region
1570
134
99
56
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Cow (Bovine)
355
342
336
233
232
122
113
87
80
72
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Rabbit
846
798
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Anti-Bovine IgG F(c) was produced by repeated immunization with bovine IgG F(c) fragment in rabbit.

Immunogen Type: Native Protein

Isotype IgG
Specificity IgG F(c)
Cross-Reactivity Cow (Bovine)
Characteristics Anti-Bovine IgG F(c) antibody generated in rabbit detects specifically bovine IgG. This secondary antibody anti-Bovine is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Sterility Sterile filtered
Antigen
Background

Synonyms: rabbit anti-Bovine IgG F(c) Antibody, Rb-a-Bovine IgG F(c), Bovine IgG F(c) Antibody in Rabbit, Rabbit IgG F(c) Secondary Antibody.

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.

Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Bovine IgG F(c) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions For Research Use only
Format Liquid
Concentration 2.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for Rabbit anti-Cow (Bovine) IgG (Fc Region) antibody - Preadsorbed (ABIN100938) ELISA results of purified Rabbit anti-Bovine IgG F(c) Antibody tested against purifie...