Rabbit anti-Cat (Feline) IgG (Heavy & Light Chain) Antibody (HRP)

Details for Product No. ABIN100964
Antigen
Epitope
Heavy & Light Chain
1569
134
100
56
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Cat (Feline)
355
342
336
233
232
122
113
87
80
73
72
65
61
56
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Rabbit
846
798
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Conjugate
HRP
235
220
209
150
147
130
75
49
48
35
31
26
26
26
26
25
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
16
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Cat IgG whole molecule

Isotype IgG
Specificity IgG (H&L)
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Cat IgG and Cat Serum.
Antigen
Background

Synonyms: rabbit anti-Cat IgG Antibody Peroxidase Conjugated, rabbit anti-Cat IgG HRP Conjugated Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Cat IgG (H&L) Peroxidase antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.

Application Notes

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

ELISA Dilution: 1:10,000 - 1:50,000

Western Blot Dilution: 1:1,000 - 1:10,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 10.0 mg/mL
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: None

Preservative Without preservative
Handling Advice Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage RT,4 °C,-20 °C
Expiry Date 12 months