Goat anti-Chicken IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Details for Product No. ABIN101007
Antigen
Epitope
Heavy & Light Chain
1569
134
100
56
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Chicken
355
342
336
233
232
122
113
87
79
73
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Goat
845
799
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Conjugate
Alkaline Phosphatase (AP)
236
220
209
149
147
130
75
49
48
35
31
26
26
26
26
25
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
16
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Chicken IgG whole molecule
Isotype IgG
Specificity IgG (H&L)
Characteristics Anti-Chicken IgG (H&L) Antibody generated in goats detects specifically Chicken IgG whole molecule. Anti-Chicken IgG (H&L) is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Chicken IgG (H&L) Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Sterility Sterile filtered
Antigen
Background

Synonyms: Goat anti-Chicken IgG Antibody Alkaline Phosphatase Conjugation, Goat anti-Chicken IgG Alk Phos Conjugated Antibody

Background: Anti-Chicken IgG Alkaline Phosphatase Antibody generated in goat detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Anti-Chicken IgG (H&L) Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Application Notes

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Chicken IgG (H&L) antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:2,000 - 1:10,000

Western Blot Dilution: 1:500 - 1:2,500
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.1 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for Goat anti-Chicken IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN101007) Western Blot of Anti-Chicken IgG (H&L) (GOAT) Antibody . Lane M: 3 μl Molecular Ladde...