Rabbit anti-Chicken IgG (Heavy & Light Chain) Antibody - Preadsorbed

Details for Product No. ABIN101033
Antigen
Epitope
Heavy & Light Chain
1569
134
100
56
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Chicken
355
342
336
233
232
122
113
87
79
73
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Rabbit
846
798
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Anti-Chicken IgG (H&L) was produced by repeated immunization with Chicken IgG whole molecule in rabbit.

Immunogen Type: Native Protein

Isotype IgG
Specificity IgG (H&L)
Cross-Reactivity Chicken
Characteristics Anti-Chicken IgG antibody generated in rabbit detects specifically chicken IgG. This secondary antibody anti-Chicken is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: immunoaffinity chromatography using Chicken IgG coupled to agarose beads
Sterility Sterile filtered
Antigen
Background

Synonyms: Rabbit anti-Chicken IgG Antibody, Chicken Secondary Antibody

Background: Anti-Chicken IgG Antibody generated in rabbit detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Chicken IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

ELISA Dilution: 1:200,000 - 1:400,000

Western Blot Dilution: 1:10,000 - 1:30,000

Restrictions For Research Use only
Format Liquid
Concentration 10 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for Rabbit anti-Chicken IgG (Heavy & Light Chain) antibody - Preadsorbed (ABIN101033) Western Blot of Anti-Chicken IgG (H&L) (RABBIT) Antibody . Lane M: 3 μl Molecular Lad...
Image no. 2 for Rabbit anti-Chicken IgG (Heavy & Light Chain) antibody - Preadsorbed (ABIN101033) Western Blot of Rabbit anti-Chicken IgG Antibody. Lane 1: Chicken IgG. Lane 2: None. ...