Rabbit anti-Donkey IgG (Heavy & Light Chain) Antibody
-
- Target See all IgG products
- IgG
-
Binding Specificity
- Heavy & Light Chain
-
Reactivity
- Donkey
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 616-4102
- Supplier
- Rockland
- Purpose
- Donkey IgG (H&L) Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Donkey IgG, and Donkey Serum.
- Characteristics
- Anti-Donkey IgG Rhodamine Antibody generated in rabbit detects donkey IgG.
- Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Sterility
- Sterile filtered
- Immunogen
- Optional[Immunogen]: Donkey IgG whole molecule
- Isotype
- IgG
-
-
- Application Notes
- Application Note: Anti-Donkey IgG antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 2.239 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
-