Rabbit anti-Golden Syrian Hamster IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN101372

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Golden Syrian Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG (H&L)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Hamster IgG and Hamster Serum.

Immunogen:

Immunogen: Hamster IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

ELISA Dilution: 1:10,000 - 1:50,000

Western Blot Dilution: 1:1,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: None

Preservative: Without preservative

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Rabbit Anti-Golden Syrian Hamster IgG Antibody Peroxidase Conjugated, Rabbit Anti-Golden Syrian Hamster IgG Peroxidase Conjugated Antibody, Rabbit Anti-Hamster IgG antibody HRP Conjugated, Rabbit Anti-Hamster IgG HRP Conjugated Antibody

Background: Anti-Golden Syrian Hamster IgG Peroxidase Antibody generated in rabbit detects Golden Syrian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.