Chicken anti-Human IgG (Heavy & Light Chain) Antibody - Preadsorbed

Details for Product No. ABIN101468
Antigen
Epitope
Heavy & Light Chain
1569
134
100
56
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Human
355
341
336
233
232
122
113
87
80
73
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Chicken
846
799
335
169
92
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Options
Immunogen

Immunogen: Human IgG whole molecule

Isotype IgG
Specificity IgG (H&L)
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Sterility Sterile filtered
Antigen
Background

Synonyms: Chicken Anti Human IgG Antibody, Chicken Anti-Human IgG Antibody

Background: Anti-Human IgG (H&L) generated in chicken detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Human IgG antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

ELISA Dilution: 1:20,000 - 1:60,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions For Research Use only
Format Liquid
Concentration 1.9 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Expiry Date 12 months