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Chicken anti-Human IgG (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
Catalog No. ABIN101468
$207.90
Plus shipping costs $45.00
2 mg
Shipping to: United States
Delivery in 1 to 2 Business Days
  • Target See all IgG products
    IgG
    Binding Specificity
    • 1508
    • 136
    • 114
    • 49
    • 35
    • 30
    • 25
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    Heavy & Light Chain
    Reactivity
    • 343
    • 322
    • 299
    • 214
    • 201
    • 108
    • 105
    • 81
    • 76
    • 72
    • 69
    • 62
    • 60
    • 55
    • 54
    • 46
    • 41
    • 30
    • 27
    • 12
    • 9
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 808
    • 765
    • 306
    • 159
    • 74
    • 70
    • 22
    • 20
    • 16
    • 9
    • 5
    • 4
    • 1
    • 1
    Chicken
    Clonality
    • 1768
    • 145
    Polyclonal
    Application
    • 1236
    • 1085
    • 906
    • 782
    • 528
    • 508
    • 501
    • 138
    • 88
    • 65
    • 61
    • 58
    • 53
    • 52
    • 28
    • 25
    • 16
    • 12
    • 11
    • 10
    • 10
    • 5
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    IgG (H&L)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Human IgG whole molecule

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Human IgG antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:20,000 - 1:60,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.9 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: Chicken Anti Human IgG Antibody, Chicken Anti-Human IgG Antibody

    Background: Anti-Human IgG (H&L) generated in chicken detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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