Rabbit anti-Sheep (Ovine) IgG (F(ab')2 Region) Antibody (FITC) - Preadsorbed

Details for Product No. ABIN102274
Antigen
Epitope
F(ab')2 Region
1572
134
101
57
34
33
25
6
6
6
6
5
4
4
4
3
3
1
1
1
Reactivity
Sheep (Ovine)
356
343
337
234
233
122
113
87
80
76
72
67
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Rabbit
847
800
337
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Conjugate
FITC
237
220
209
150
147
130
75
50
48
36
31
26
26
26
26
26
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
16
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Options
Immunogen

Immunogen: Sheep IgG F(ab')2 fragment

Isotype IgG
Specificity IgG F(ab')2
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Labeling Ratio 3.3
Antigen
Background

Synonyms: Rabbit Anti-Sheep IgG F(ab')2 fluorescein Conjugated Antibody, Rabbit Anti-Sheep IgG Fab2 fluorescein Conjugated Antibody, Rabbit Anti-Sheep IgG Fab2 Fragment Antibody FITC Conjugation

Background: Anti-Sheep IgG F(ab')2 Fluorescein Antibody generated in rabbit recognizes the dimeric Fab portion of the sheep IgG molecule. Sheep IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind sheep IgG F(c) will not bind sheep IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG F(ab')2 Antibody is conjugated to Fluorescein.

Application Notes

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Comment

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.5 mg/mL
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Product is photosensitive and should be protected from light.
Storage RT,4 °C,-20 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for Rabbit anti-Sheep (Ovine) IgG (F(ab')2 Region) antibody (FITC) - Preadsorbed (ABIN102274) Dot Blot results of Rabbit Anti-Sheep IgG F(ab')2 Antibody Fluorescein Conjugate. Dot...