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Avidin antibody (Biotin) Primary Antibody

Reactivity: Chicken EIA, IHC (fro), IHC (p), WB Host: Rabbit Polyclonal
Catalog No. ABIN116961
$2,937.00
Plus shipping costs $45.00
10 mg
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  • Target
    Reactivity
    Chicken
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This Avidin antibody is conjugated to Biotin
    Application
    Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White].
    Characteristics
    Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
    Purification
    A multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below
    Immunogen
    Avidin [Hen Egg White]
  • Application Notes
    Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. This product has been assayed against 1.0 μg of Avidin [Hen Egg White] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat)(R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 8,000 to 1: 32,000 of the reconstitution concentration is suggestedfor this product.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1.0 mL of deionized water (or equivalent).
    Concentration
    10.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. Do NOT add sodium azide! Dilute only prior to immediate use.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below.
  • Target
    Synonyms
    avidin, avd, LOC723980, AVD
    Background
    The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).Synonyms: AVD
    Gene ID
    396260
    NCBI Accession
    NP_990651
    UniProt
    P02701
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