AGO5 antibody

Catalog No. ABIN1720795

Product Details anti-AGO5 Antibody

Target: AGO5 (Argonaute Family Protein (AGO5))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Specificity: TAIR: At2g27880

Cross-Reactivity (Details): Not reactive in: Zea mays

Predicted Reactivity: Arabidopsis thaliana

Purification: serum

Immunogen: peptide of Arabidopsis thaliana AGO5 UniProt: Q9SJK3, TAIR:AT2G27880

Application Details

Application Notes: 1: 1500 (WB), 5 μg per gram floral tissue (IP)

Comment:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Handling Advice: Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for AGO5

Target: AGO5 (Argonaute Family Protein (AGO5))

Alternative Name: AGO5

Synonyms: ARGONAUTE 5 antibody, T1E2.20 antibody, T1E2_20 antibody, Argonaute family protein antibody, AGO5 antibody

Background: AGI Code: AT2G27880
AGO5 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). Probably involved in antiviral RNA silencing.

Molecular Weight: expected: 111 kDa, appearent: 111 kDa

UniProt: Q9SJK3