Mgl, Macrophage Galactose-Specific Lectin antibody
Reactivity: Mouse
IHC (fro), FACS, InhA
Host: Rat
Monoclonal
ER-MP23
unconjugated
(2 references)-
- Target
- Mgl, Macrophage Galactose-Specific Lectin
- Reactivity
- Mouse
- Host
- Rat
- Clonality
- Monoclonal
- Application
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Inhibition Assay (InhA)
- Sterility
- 0.2 μm filtered
- Clone
- ER-MP23
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- Application Notes
- For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For blocking studies in vitro, dilutions have to be made according to the amounts of MGL to be inactivated. The antigen is glutaraldehyde (0.05 % ) resistant.
- Restrictions
- For Research Use only
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- Buffer
- PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- Expiry Date
- 12 months
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: "Islet abnormalities associated with an early influx of dendritic cells and macrophages in NOD and NODscid mice." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 80, Issue 5, pp. 769-77, (2000) (PubMed).
: "Immunohistochemical characterization of monocytes-macrophages and dendritic cells involved in the initiation of the insulitis and beta-cell destruction in NOD mice." in: Diabetes, Vol. 43, Issue 5, pp. 667-75, (1994) (PubMed).
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: "Islet abnormalities associated with an early influx of dendritic cells and macrophages in NOD and NODscid mice." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 80, Issue 5, pp. 769-77, (2000) (PubMed).
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- Target
- Mgl, Macrophage Galactose-Specific Lectin
- Background
- The monoclonal antibody ER-MP23 specifically reacts with the macrophage galactose-specific lectin (MGL), a 38 kDa single chain surface glycoprotein. MGL is found on murine mature macrophages in the connective tissue neighboring epithelia of e.g. salivary glands, capsule of lymph nodes, thymus and various other organs. MGL is present on the surface of connective tissue macrophages and their precursor cells in bone marrow. Also, MGL is expressed in macrophage cell lines (e.g. J774-1.6, RAW309Cr.1 and WR19M.1). Expression levels of MGL are increased in mature macrophages. The antigen is co expressed with the antigen recognized by monoclonal antibody BM8 (HM1066). The monoclonal antibody ER-MP23 has been raised after immunization of rats with mouse macrophage cell lines. Blocking studies demonstrated that the monoclonal antibody ER-MP23 is able to block mouse MGL.
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