LARGE1 (AA 35-142) antibody

Catalog No. ABIN2749093

This anti- antibody is a Mouse Monoclonal antibody detecting in WB and FACS. Suitable for Human.

Quick Overview for LARGE1 (AA 35-142) antibody (ABIN2749093)

Target: LARGE1

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: LARGE-02

Product Details

Binding Specificity: AA 35-142

Purpose: Anti-LARGE1 Purified

Specificity: The mouse monoclonal antibody LARGE-02 recognizes human LARGE1, a glycosyltransferase expressed mainly in the Golgi apparatus. Crossreactivity with LARGE2 was not determined.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Recombinant fragment of human LARGE1 (amino acids 35-142)

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details

Target: LARGE1

Background: LARGE xylosyl- and glucuronyltransferase 1,LARGE1 serves as a glycosyltransferase which participates in glycosylation of the muscle membrane protein alpha-dystroglycan. Mutations of LARGE1 lead to hypoglycosylation of alpha-dystroglycan and cause congenital muscular dystrophy (MDC1D) associated with severe mental retardation. Altered alpha-dystroglycan glycosylation may also play a role in cancer, as hypoglycosylation of the protein and loss of laminin binding have been demonstrated in invasive carcinoma cells.,Xysosyl- and glucosyl transferase 1

Gene ID: 9215

UniProt: O95461