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HYAL1 antibody (Hyaluronidase-1) (N-Term) Primary Antibody

HYAL1 Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit IHC, WB Host: Rabbit Polyclonal unconjugated
Independent Validation (1)
Catalog No. ABIN2784343
$389.00
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100 μL
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  • Target
    HYAL1
    Binding Specificity
    • 17
    • 15
    • 9
    • 8
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 71
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
    Host
    • 63
    • 7
    • 4
    Rabbit
    Clonality
    • 73
    • 1
    Polyclonal
    Conjugate
    • 30
    • 8
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This HYAL1 antibody is un-conjugated
    Application
    • 45
    • 33
    • 31
    • 13
    • 13
    • 9
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Western Blotting (WB)
    Sequence
    TIFYSSQLGT YPYYTPTGEP VFGGLPQNAS LIAHLARTFQ DILAAIPAPD
    Predicted Reactivity
    Cow: 86%, Dog: 85%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 92%
    Characteristics
    This is a rabbit polyclonal antibody against HYAL1. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N terminal region of human HYAL1
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 435 AA

    Restrictions
    For Research Use only
  • Validation #100034 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    University of Manitoba, Max Rady College of Medicine Biochemistry and Medical Genetics, Department of Biochemistry and Medical Genetics
    No.
    #100034
    Date
    08/16/2016
    Antigen
    HYAL1 antibody - N-terminal region
    Lot Number
    QC23783
    Method validated
    Immunohistochemistry
    Positive Control
    Testes of Hyal1 +/+ mouse
    Negative Control
    Testes of Hyal1 -/- mouse
    Notes
    ABIN2784343 does detect mouse HYAL1 by immunohistochemistry in paraffin sections. The immunohistochemistry is based on increased signal in wild type mouse testes compared to Hyal1-/- mouse testes.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN2784343
    Secondary Antibody
    Goat-anti-rabbit AF568
    Full Protocol
    • Deparafinize and rehydrate:
      • xylene (I & II) – 2x 5min
      • 100% Ethanol (I & II) – 2x 5min
      • 95% Ethanol (I & II) – 2x 3min
      • 70% Ethanol (I) – 3min
      • 50% Ethanol (I) – 3min
      • ddH2O – 3min
    • Antigen retrieval:
      • Immerse slides in citrate buffer (10mM Sodium citrate at pH6.0) preheated to 95°C.
      • Incubate at 95°C for 20min.
      • Cool down at RT for 20min.
      • Wash twice with PBS for 5min.
    • Immunostaining:
      • Block in blocking solution (prepare 3% BSA in TBST) at RT for 60min.
      • Incubate sections with primary Hyaluronidase-1 (HYAL1) (N-Term) antibody ABIN2784343 diluted 1:200 in blocking solution.
      • Incubate at 4°C overnight.
      • Wash slides three times with TBST 5min.
      • Incubate sections with goat-anti-rabbit AF568 secondary antibody conjugate diluted 1:500 in blocking solution.
      • Incubate at RT for 1h.
      • Wash slides in TBST - 1x 5min at RT.
      • Wash three times with H2O for 5min.
      • Incubated in Hoechst solution diluted 1:100.000 for 8-9min.
      • Wash twice with H2O for 5min.
      • Mounted with ProLong Gold (ThermoFisher, P36931) solution.
    Experimental Notes
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    HYAL1
    Alternative Name
    HYAL1 (HYAL1 Antibody Abstract)
    Synonyms
    HYAL-1, LUCA1, NAT6, Hya1, Hyal-1, hyaluronoglucosaminidase 1, HYAL1, Hyal1
    Background
    HYAL1 is a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.
    Alias Symbols: HYAL-1, LUCA1, MGC45987, NAT6
    Protein Interaction Partner: UBC, COL2A1,
    Protein Size: 435
    Molecular Weight
    45 kDa
    Gene ID
    3373
    NCBI Accession
    NM_153282, NP_695014
    UniProt
    Q12794
    Pathways
    Glycosaminoglycan Metabolic Process
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