HYAL1 antibody (Hyaluronidase-1) (N-Term)

Catalog No. ABIN2784343

Product Details anti-HYAL1 Antibody

Target: HYAL1

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HYAL1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Sequence: TIFYSSQLGT YPYYTPTGEP VFGGLPQNAS LIAHLARTFQ DILAAIPAPD

Predicted Reactivity: Cow: 86%, Dog: 85%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against HYAL1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HYAL1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 435 AA

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #100034 (Immunohistochemistry)

by: University of Manitoba, Max Rady College of Medicine Biochemistry and Medical Genetics, Department of Biochemistry and Medical Genetics

No.: #100034

Date: 08/16/2016

Antigen: HYAL1 antibody - N-terminal region

Lot Number: QC23783

Method validated: Immunohistochemistry

Positive Control: Testes of Hyal1 +/+ mouse

Negative Control: Testes of Hyal1 -/- mouse

Notes: ABIN2784343 does detect mouse HYAL1 by immunohistochemistry in paraffin sections. The immunohistochemistry is based on increased signal in wild type mouse testes compared to Hyal1-/- mouse testes.

Validation Images

Testes of Hyal1 -/- mouse (A) or Hyal1 +/+ mouse (B) stained with ABIN2784342 as described in the protocol.

Full Methods

Primary Antibody: ABIN2784343

Secondary Antibody: Goat-anti-rabbit AF568

Full Protocol:

• Deparafinize and rehydrate:
• xylene (I & II) – 2x 5min
• 100% Ethanol (I & II) – 2x 5min
• 95% Ethanol (I & II) – 2x 3min
• 70% Ethanol (I) – 3min
• 50% Ethanol (I) – 3min
• ddH₂O – 3min
• Antigen retrieval:
• Immerse slides in citrate buffer (10mM Sodium citrate at pH6.0) preheated to 95°C.
• Incubate at 95°C for 20min.
• Cool down at RT for 20min.
• Wash twice with PBS for 5min.
• Immunostaining:
• Block in blocking solution (prepare 3% BSA in TBST) at RT for 60min.
• Incubate sections with primary Hyaluronidase-1 (HYAL1) (N-Term) antibody ABIN2784343 diluted 1:200 in blocking solution.
• Incubate at 4°C overnight.
• Wash slides three times with TBST 5min.
• Incubate sections with goat-anti-rabbit AF568 secondary antibody conjugate diluted 1:500 in blocking solution.
• Incubate at RT for 1h.
• Wash slides in TBST - 1x 5min at RT.
• Wash three times with H₂O for 5min.
• Incubated in Hoechst solution diluted 1:100.000 for 8-9min.
• Wash twice with H₂O for 5min.
• Mounted with ProLong Gold (ThermoFisher, P36931) solution.

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HYAL1

Target: HYAL1

Alternative Name: HYAL1 (HYAL1 Antibody Abstract)

Synonyms: HYAL-1, LUCA1, NAT6, Hya1, Hyal-1, hyaluronoglucosaminidase 1, HYAL1, Hyal1

Background: HYAL1 is a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: HYAL-1, LUCA1, MGC45987, NAT6
Protein Interaction Partner: UBC, COL2A1,
Protein Size: 435

Molecular Weight: 45 kDa

Gene ID: 3373

NCBI Accession: NM_153282, NP_695014

UniProt: Q12794

Pathways: Glycosaminoglycan Metabolic Process