Dinitrophenyl (DNP) is a hapten that is often used for labeling primary or secondary probes in immunological assays. Therefore, anti-DNP antibody is a useful tool for the detection and analysis of target molecules. Monoclonal Anti-DNP (Anti-Hapten) antibody, Mouse IgG1 was purified from HEK293 cell culture at ACRObiosystems, and specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
Purification
Protein A affinity chromatography from HEK293 supernatants.
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally mannitol or trehalose are added as protectants before lyophilization.
Storage
4 °C/-80 °C
Storage Comment
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:4 ℃ for 1 year in lyophilized state.-70 ℃ for 3 months under sterile conditions after reconstitution.
Liu, Zhang, Wang, Haymour, Zhang, Xu, Srinivasarao, Low: "A universal dual mechanism immunotherapy for the treatment of influenza virus infections." in: Nature communications, Vol. 11, Issue 1, pp. 5597, (2020) (PubMed).
A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.