TRPV4 antibody (Transient Receptor Potential Cation Channel, Subfamily V, Member 4) (AA 300-400)

Details for Product anti-TRPV4 Antibody No. ABIN351285
Antigen
  • wu:fp52e02
  • CMT2C
  • HMSN2C
  • OTRPC4
  • SMAL
  • SPSMA
  • SSQTL1
  • TRP12
  • VRL2
  • VROAC
  • 0610033B08Rik
  • Trp12
  • VR-OAC
  • VRL-2
  • Otrpc4
  • Vroac
  • transient receptor potential cation channel, subfamily V, member 4
  • transient receptor potential cation channel subfamily V member 4
  • trpv4
  • TRPV4
  • Trpv4
Epitope
AA 300-400
16
14
8
7
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Mouse (Murine), Human, Rat (Rattus)
106
64
62
7
7
6
4
3
2
2
2
1
1
Host
Rabbit
109
6
1
Clonality
Polyclonal
Conjugate
This TRPV4 antibody is un-conjugated
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
82
41
39
14
13
6
2
1
1
Options
Immunogen A synthetic peptide from aa region 300-400 of mouse TRPV4 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and human.
Isotype IgG
Specificity Specific for TRPV4.
Plasmids, Primers & others Plasmids, Primers & others TRPV4 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name TRPV4 (TRPV4 Antibody Abstract)
Background TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure.
Function: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.
Subunit: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES.
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia. SIMILARITY: Belongs to the transient receptor family, TrpV subfamily. SIMILARITY: Contains 3 ANK repeats. Also known as: Transient receptor potential cation channel subfamily V member 4, TrpV4, osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Transient receptor potential protein 12, TRP12, Trp12, VRL-2, VROAC, OTRPC4, VR-OAC, MGC150050, 0610033B08Rik, Trp12, Vrl2, Vroac.
Pathways Hormone Transport, Cell-Cell Junction Organization
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other application.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Arniges, Fernuandez-Fernuandez, Albrecht, Schaefer, Valverde: "Human TRPV4 channel splice variants revealed a key role of ankyrin domains in multimerization and trafficking." in: The Journal of biological chemistry, Vol. 281, Issue 3, pp. 1580-6, 2006 (PubMed).

Earley, Heppner, Nelson, Brayden: "TRPV4 forms a novel Ca2+ signaling complex with ryanodine receptors and BKCa channels." in: Circulation research, Vol. 97, Issue 12, pp. 1270-9, 2005 (PubMed).

Strotmann, Schultz, Plant: "Ca2+-dependent potentiation of the nonselective cation channel TRPV4 is mediated by a C-terminal calmodulin binding site." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 26541-9, 2003 (PubMed).

Liedtke, Choe, Martí-Renom, Bell, Denis, Sali, Hudspeth, Friedman, Heller: "Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor." in: Cell, Vol. 103, Issue 3, pp. 525-35, 2000 (PubMed).

Strotmann, Harteneck, Nunnenmacher, Schultz, Plant: "OTRPC4, a nonselective cation channel that confers sensitivity to extracellular osmolarity." in: Nature cell biology, Vol. 2, Issue 10, pp. 695-702, 2000 (PubMed).

Wissenbach, Bödding, Freichel, Flockerzi: "Trp12, a novel Trp related protein from kidney." in: FEBS letters, Vol. 485, Issue 2-3, pp. 127-34, 2000 (PubMed).

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