Goat anti-Human IgM Antibody (FITC)

Details for Product No. ABIN376129
Antigen
Reactivity
Human
160
89
46
21
20
16
14
11
11
9
8
8
8
7
7
5
2
2
1
1
Host
Goat
218
105
75
11
11
5
4
2
1
1
1
Clonality
Polyclonal
Conjugate
FITC
54
46
43
25
23
20
17
9
7
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS)
Options
Isotype IgG
Fragment F(ab')2 fragment
Specificity Reacts with the heavy chain of human IgM
Characteristics Goat F(ab')2 Anti-Human IgM-FITC
Purification Affinity chromatography on hamster IgG covalently linked to agarose. F(ab') 2 fragment prepared by pepsin digestion of affinity purified IgG.
Antigen
Application Notes
  • Applications: Quality tested applications include - ELISA , FLISA FC ,
  • Other referenced applications include - ICC , ELISPOT , Stim
  • Working Dilutions: ELISA HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Comment

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer 0.5 mg in 1.0 mL of PBS/Sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Storage Comment Store at 2-8°C
Supplier Images
Image no. 1 for Goat anti-Human IgM antibody (FITC) (ABIN376129) FLISA plate was coated with purified human IgM, IgG, and IgA. Immunoglobulins were d...