HPV-18 E2 (AA 1-83) antibody

Details for Product No. ABIN457548
Antigen
Epitope
AA 1-83
Reactivity
Human Papillomavirus (HPV)
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
Options
'Independent Validation' Badge
Antigen HPV-18
Lot Number A10P2E71-001
Method validated Unfolding Profile
Positive Control ABIN457548
Notes

Passed. ABIN457548 showed a clear unfolding profile with two detected unfolding events with Tis at 78.4°C and 84.4°C. It is not unusual for antibodies to show more than one unfolding transition, as they are composed of distinct domains that may unfold independently and at different temperatures. This result suggests that the protein is properly folded and functional.

Primary Antibody ABIN457548
Protocol
  • Dilute ABIN457548 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 0.67µM.
  • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
  • Run Tycho measurement.
Experimental Notes

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Validation Images
Unfolding Profile validation image for anti-HPV-18 E2 (AA 1-83) antibody (ABIN457548) Unfolding profile of ABIN457548. The fluorescence signal is plotted against temperatu...
Immunogen Recombinant full-length HPV-18 E2-protein purifiedfrom E. coli
Clone 2E7
Isotype IgG
Specificity Reacts with HPV-18 E2-protein.
Purification purified with Protein G affinity chromatorgaphy
Antigen
Background Human papillomaviruses(HPV) are small DNA viruses whichinfect epithelia of the skin and mucosa. Over 90 types have been identified andthey mostly cause a variety of benign lesions such as warts and verrucae.However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmedas agents which cause cervical cancer. Human Papillomavirus (HPV) E7 proteins havecellular transforming and trans-activating activities
Application Notes ELISA:1: 2 000, WB: 1: 1000. This antibody does not work in IP. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/ml
Buffer PBS pH 7.4, with 0.1%
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.
Storage -20 °C
Storage Comment This product is shipped in non-frozen liquid form in ambient conditions
Supplier Images
Image no. 1 for anti-HPV-18 E2 (AA 1-83) antibody (ABIN457548) IF analysis of HPV-18 E2 protein in U2OS cells. IF was carried out with Hela cells tr...
Image no. 2 for anti-HPV-18 E2 (AA 1-83) antibody (ABIN457548) Western-Blot analysis of HPV-18 E2 protein. WB was was carried out with 2E7 Mab using...
Background publications Kadaja, Sumerina, Verst, Ojarand, Ustav, Ustav: "Genomic instability of the host cell induced by the human papillomavirus replication machinery." in: The EMBO journal, Vol. 26, Issue 8, pp. 2180-91, 2007 (PubMed).

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