CPK antibody (Creatine Phosphokinase)

Details for Product anti-CPK Antibody No. ABIN458973
Target
Reactivity
Rabbit
18
6
Host
Sheep
18
6
Clonality
Polyclonal
Conjugate
This CPK antibody is un-conjugated
4
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
22
16
16
7
5
2
2
2
2
2
2
1
Options
Immunogen Creatine phosphokinase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity Cross-reactivities against enzymes of other sources may occur but have not been determined.
Characteristics Affinity purified antibodies of polyclonal sheep antiserum to creatine phosphokinase from rabbit muscle
Purification Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.
Target
Alternative Name Creatine Phosphokinase
Background The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Application Notes This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
Restrictions For Research Use only
Format Lyophilized
Concentration PAb concentration 1 mg/0.5ml. No foreign proteins added.
Buffer Purified hyperimmune sheep IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.