ACE2 antibody (Angiotensin I Converting Enzyme 2) (Internal Region)

Details for Product anti-ACE2 Antibody No. ABIN461888
Target
Binding Specificity
Internal Region
20
16
12
11
11
10
9
8
8
8
7
5
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
180
50
39
19
2
2
2
2
1
1
Host
Rabbit
161
30
4
Clonality
Polyclonal
Conjugate
This ACE2 antibody is un-conjugated
25
13
10
9
9
8
3
3
3
3
3
3
3
2
1
1
1
1
Application
ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
157
93
72
44
30
13
9
7
5
5
4
2
1
1
1
Options
Brand IHC-plus™
Isotype IgG
Specificity Anti-ACE2 has no cross response to ACE1.
Purification Immunoaffinity purified
Target
Alternative Name ACE2 / ACE-2 (ACE2 Antibody Abstract)
Background Name/Gene ID: ACE2
Subfamily: Metallopeptidase M2
Family: Protease

Synonyms: ACE2, ACE-related carboxypeptidase, ACEH, Metalloprotease MPROT15
Gene ID 59272
UniProt Q9BYF1
Pathways ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
Application Notes Approved: ELISA, IHC, IHC-P (2.5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot: ACE2 antibody can be used for the detection of ACE2 by Western blot at 0.5 to 2 μg/mL. A 90 kD band can be detected. Anti-ACE2 has no cross response to ACE1.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles. Store undiluted.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months
Images
Image no. 1 for anti-Angiotensin I Converting Enzyme 2 (ACE2) (Internal Region) antibody (ABIN461888) Human Testis (formalin-fixed, paraffin-embedded) stained with ACE2 antibody ABIN46188...
Image no. 2 for anti-Angiotensin I Converting Enzyme 2 (ACE2) (Internal Region) antibody (ABIN461888) Human Kidney (formalin-fixed, paraffin-embedded) stained with ACE2 antibody ABIN46188...
Image no. 3 for anti-Angiotensin I Converting Enzyme 2 (ACE2) (Internal Region) antibody (ABIN461888) Anti-ACE-2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, para...