LC3A / LC3B antibody

Catalog No. ABIN4953268

Product Details

Target: LC3A / LC3B

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Purification: Protein G affinity

Immunogen: A synthetic LC3B peptide was used as the immunogen for this LC3A/B antibody.

Clone: 3E9-E5-C9

Isotype: IgG2b

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the LC3A/B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 50 % Glycerol and 0.03 % ProClin 300

Storage: -20 °C

Storage Comment: Store the LC3A/B antibody at -20°C.

Target Details

Target: LC3A / LC3B

Alternative Name: LC3A/B

Background: LC3A:Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. [UniProt]

LC3B:Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. [UniProt]

UniProt: Q9H492, Q9GZQ8