CD31 antibody
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- Target See all CD31 (PECAM1) Antibodies
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD31 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purification
- Protein G affinity chromatography
- Immunogen
- Recombinant human protein was used as the immunogen for this CD31 antibody.
- Clone
- PCM25-1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PECAM1 Primary Antibody
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- Application Notes
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Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/10e6 cells
IF: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the CD31 antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
- Alternative Name
- CD31 (PECAM1 Products)
- Background
- CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]
- Gene ID
- 5175
- UniProt
- P16284
- Pathways
- Regulation of Actin Filament Polymerization
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