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CD31 antibody

PECAM1 Reactivity: Human FACS, WB, IHC, IF Host: Mouse Monoclonal PCM25-1 unconjugated
Catalog No. ABIN6252198
  • Target See all CD31 (PECAM1) Antibodies
    CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
    Reactivity
    • 264
    • 136
    • 74
    • 59
    • 43
    • 31
    • 21
    • 20
    • 11
    • 7
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    Human
    Host
    • 156
    • 140
    • 42
    • 8
    • 6
    • 3
    • 2
    Mouse
    Clonality
    • 221
    • 127
    • 8
    • 1
    Monoclonal
    Conjugate
    • 156
    • 30
    • 30
    • 24
    • 13
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This CD31 antibody is un-conjugated
    Application
    • 229
    • 177
    • 104
    • 98
    • 60
    • 36
    • 35
    • 34
    • 30
    • 26
    • 18
    • 15
    • 13
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    • 9
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
    Purification
    Protein G affinity chromatography
    Immunogen
    Recombinant human protein was used as the immunogen for this CD31 antibody.
    Clone
    PCM25-1
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product PECAM1 Primary Antibody
  • Application Notes
    Western blot: 1-2 μg/mL

    Flow Cytometry: 0.5-1 μg/10e6 cells

    IF: 1-2 μg/mL

    IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    Titering of the CD31 antibody may be required for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Buffer
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • Target
    CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
    Alternative Name
    CD31 (PECAM1 Products)
    Background
    CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]
    Gene ID
    5175
    UniProt
    P16284
    Pathways
    Regulation of Actin Filament Polymerization
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