KRT17 antibody

Catalog No. ABIN6297731

Product Details anti-KRT17 Antibody

Target: KRT17 (Keratin 17 (KRT17))

Reactivity: Human, Rat, Cow, Pig, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT17 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Mouse anti-Human/Rat/Cow/Goat/Pig Cytokeratin 17 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: The cytoskeletal fraction of rat colon epithelium was used as the immunogen for this Cytokeratin 17 antibody.

Immunogen: The cytoskeletal fraction of rat colon epithelium was used as the immunogen for this Cytokeratin 17 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for KRT17

Target: KRT17 (Keratin 17 (KRT17))

Alternative Name: Keratin, type I cytoskeletal 17 (KRT17 Products)

Synonyms: K17 antibody, Krt1-17 antibody, PC antibody, PC2 antibody, PCHC1 antibody, Ka17 antibody, KRT17 antibody, krt16 antibody, si:dkeyp-113d7.7 antibody, KRT14 antibody, keratin 17 antibody, keratin 17 L homeolog antibody, keratin 17, type I antibody, keratin, type I cytoskeletal 17 antibody, Krt17 antibody, KRT17 antibody, krt17 antibody, krt17.L antibody, LOC100737113 antibody, LOC102177275 antibody

Background:

Target Description: Cytokeratin 17 (CK17, Keratin 17) is a member of the Cytokeratin subfamily of intermediate filament proteins (IFP 's). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Cytokeratin 17 is expressed in the nail bed, hair follicle, sebaceous glands and other epidermal appendages. Antibody to cytokeratin 17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary. It is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered as ""epithelial stem cell "" marker because cytokeratin 17 antibody marks basal cell differentiation. Cytokeratin 17 can be useful when included in a panel of antibodies against TTF-1, napsin A, CK5&6, p63, and SOX-2 for diagnostic differentiation between lung adenocarcinoma (LADC) and lung squamous cell carcinoma (SCLC), especially for poorly-differentiated lung carcinoma. Cytokeratin 17 is expressed in SCLC much higher than in LADC. In breast carcinomas, approximately 20 % of patients show no expression of ER, PR and Her2, which are defined as triple negative tumor. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including cytokeratin 17 antibody. Also important is that cases of triple negative breast carcinoma with expression of keratin 17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually antibody to cytokeratin 17 and MUC1 immunoreactivity represents pancreatobiliary subtype whereas antibody to MUC2 and CDX2 positivity defines intestinal subtype.

Gene Symbol: KRT17

Gene ID: 3872

UniProt: Q04695