MecA PBP2 antibody (Biotin)
MecA PBP2
Reactivity: Human
ELISA, WB
Host: Mouse
Monoclonal
5B9-G2-F12
Biotin
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- Target See all MecA PBP2 products
- MecA PBP2 (Penicillin Binding Protein 2a (MecA PBP2))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MecA PBP2 antibody is conjugated to Biotin
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Application
- ELISA, Western Blotting (WB)
- Purpose
- Mouse anti-Human PBP2a Biotinylated Antibody
- Immunogen
- This antibody was produced by fusion of mouse myeloma with B cells obtained from a mouse immunized with a recombinant protein immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
- Clone
- 5B9-G2-F12
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- Application Notes
- ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000), (see image) Western Blot: First Antibody: 1µ,g/mL (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
- Restrictions
- For Research Use only
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- Reconstitution
- A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e. g. sodium azide). The final amount is indicated on the shipping vial.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C
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- Target
- MecA PBP2 (Penicillin Binding Protein 2a (MecA PBP2))
- Alternative Name
- PBP2a (MecA PBP2 Products)
- Background
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Target Description: PBP2A has a low affinity for beta-lactam antibiotics such as methicillin and penicillin. This enables transpeptidase activity in the presence of beta-lactams, preventing them from inhibiting cell wall synthesis.
- UniProt
- Q53707
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