PBP2a antibody (Biotin)

Details for Product No. ABIN6297798
Antigen
Reactivity
Human
5
2
Host
Mouse
6
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
2
Application
ELISA, Western Blotting (WB)
7
7
2
2
2
2
Options
Purpose Mouse anti-Human PBP2a Biotinylated Antibody
Immunogen This antibody was produced by fusion of mouse myeloma with B cells obtained from a mouse immunized with a recombinant protein immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Clone 5B9-G2-F12
Antigen
Background

Target Description: PBP2A has a low affinity for beta-lactam antibiotics such as methicillin and penicillin. This enables transpeptidase activity in the presence of beta-lactams, preventing them from inhibiting cell wall synthesis.

UniProt Q53707
Application Notes ELISA (recommended dilution= 1:320,000), Western Blotting (recommended dilution= 1:1,000-5,000), (see image) Western Blot: First Antibody: 1µ,g/mL (Mouse anti-PBP2a (1X1000)), Second Antibody: 12,000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ,g Lane 2: MSSA lysate 15µ,l
Restrictions For Research Use only
Reconstitution A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e. g. sodium azide). The final amount is indicated on the shipping vial.
Storage 4 °C,-20 °C
Storage Comment -20°C
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