Dinitrophenol antibody

Catalog No. ABIN636352

Product Details anti-Dinitrophenol Antibody

Target: Dinitrophenol (DNP)

Host: Goat

Clonality: Polyclonal

Conjugate: This Dinitrophenol antibody is un-conjugated

Application: Western Blotting (WB)

Immunogen: DNP antibody was raised in goat using Dinitrophenol (DNP) conjugate as the immunogen.

Isotype: IgG

Application Details

Application Notes: WB: 1:2,000
Optimal conditions should be determined by the investigator.

Comment:

This antibody does not react with its immunogens carrier.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as serum.

Precaution of Use: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.

Target Details for Dinitrophenol

Target: Dinitrophenol (DNP)

Alternative Name: DNP (DNP Products)

Target Type: Chemical

Background: Tissues are continually exposed to reactive oxygen species (ROS) that are produced in tissues from metabolism processes or the inflammatory response of leukocytes or macrophages. These ROS can cause damage to various cellular components. Dinitrophenylhydrazine (DNPH) will react with free carbonyl groups on proteins, leading to adduction of the dinitrophenyl (DNP) group to the carbonyl group. Using an antibody to DNP, it is possible to visualize and localize the sites of oxidative damage by immunodetection of DNP adduction resulting from reaction of the tissue with DNPH.