Rabbit anti-Horse IgG Antibody (Cy3)

Catalog No. ABIN6698832

Supplier Product No.: 708-404-002

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Reactivity: Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Cy3

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 708-404-002

Supplier: Rockland

Purpose: F(ab')2 Horse IgG (H&L) Antibody Cy3 Conjugated

Characteristics: Rabbit F(ab')2 Anti-Horse IgG Antibody CY3 Conjugation, Rabbit F(ab')2 Anti-Horse IgG CY3 Conjugated Antibody, Rabbit Fab2 Anti-Horse IgG CY3 Conjugated Antibody,F(ab')2 Anti-Horse IgG Cy3 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Immunogen: Horse IgG whole molecule

Isotype: IgG

Labeling Ratio: 5.1

Application Details

Application Notes:

FLISA_Dilution: 1:10,000 - 1:50,000

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Other: FLOW CYTOMETRY 1:500 - 1:2,500

Comment:

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 250 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.