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Rabbit anti-Human IgG Antibody (DyLight 800) - Preadsorbed Secondary Antibody

FLISA, FM, WB Polyclonal IgG DyLight 800
Catalog No. ABIN6698938
$95.00
Plus shipping costs $45.00
100 μg ABIN6698938
100 μg ABIN6698938
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  • Target
    IgG
    Reactivity
    Human
    • 366
    • 335
    • 315
    • 224
    • 215
    • 111
    • 110
    • 83
    • 76
    • 73
    • 72
    • 65
    • 61
    • 56
    • 54
    • 49
    • 42
    • 31
    • 27
    • 15
    • 9
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Host
    Rabbit
    • 831
    • 780
    • 313
    • 178
    • 76
    • 75
    • 22
    • 20
    • 16
    • 13
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Clonality
    Polyclonal
    • 1796
    • 177
    Conjugate
    DyLight 800
    Application
    FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
    Characteristics

    Synonyms: rabbit anti-Human IgG Antibody DyLight™800 Conjugation, rabbit anti-Human IgG DyLight™ 800 Conjugated Antibody

    Background: Anti-Human IgG (H&L) DyLight 800 generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

    Purification
    Preadsorption: immunoaffinity chromatography using Human antigens coupled to agarose beads
    Immunogen

    Immunogen: Human IgG, whole molecule

    Isotype
    IgG
    Labeling Ratio
    2.3
  • Application Notes

    Application Note: Anti-Human IgG (H&L) DyLight 800 is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: > 1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    IgG
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