Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Sheep anti-Mouse IgG Antibody (DyLight 405) - Preadsorbed Secondary Antibody

FLISA, FM, WB Polyclonal IgG DyLight 405
Catalog No. ABIN6698991
$95.00
Plus shipping costs $45.00
100 μg
local_shipping Shipping to: United States
Delivery in 1 to 2 Business Days
  • Target
    IgG
    Reactivity
    Mouse
    • 367
    • 334
    • 315
    • 224
    • 214
    • 111
    • 110
    • 82
    • 76
    • 73
    • 72
    • 65
    • 61
    • 56
    • 54
    • 49
    • 42
    • 31
    • 27
    • 15
    • 9
    • 9
    • 7
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Host
    Sheep
    • 830
    • 781
    • 312
    • 178
    • 76
    • 74
    • 22
    • 20
    • 16
    • 13
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Clonality
    Polyclonal
    • 1795
    • 177
    Conjugate
    DyLight 405
    Application
    FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
    Characteristics

    Synonyms: Sheep Anti Mouse IgG (H&L) Antibody DyLight™ 405 Conjugated, Sheep Anti-Mouse IgG Antibody DyLight™ 405 Conjugated

    Background: Anti-Mouse IgG DyLight 405 Antibody generated in sheep detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Mouse IgG whole molecule

    Isotype
    IgG
    Labeling Ratio
    2.0
  • Application Notes

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    IgG
You are here:
help Support