Goat anti-Mouse (Murine) IgM (Chain mu) Antibody (DyLight 488) - Preadsorbed

Details for Product No. ABIN6699084
Antigen
Epitope
Chain mu
102
82
50
36
20
7
1
1
Reactivity
Mouse (Murine)
162
88
46
21
20
16
14
12
11
9
8
8
8
7
7
5
2
2
1
1
Host
Goat
219
105
75
11
11
5
4
2
1
1
1
Clonality
Polyclonal
Conjugate
DyLight 488
55
47
43
25
23
20
17
9
7
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Options
Immunogen

Immunogen: Mouse IgM whole molecule

Isotype IgG
Characteristics

Synonyms: Goat Anti-Mouse IgM (mu chain) Antibody DyLight 488™ Conjugated, Goat Anti Mouse IgM mu chain DyLight 488™ Conjugated Antibody

Background: Anti-Mouse IgM DyLight antibody specifically detects mouse IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

Purification Preadsorption: Solid phase absorption
Labeling Ratio 4.0
Antigen
Application Notes

Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiry Date 12 months
Product cited in: Nadanaka, Kagiyama, Kitagawa: "Roles of EXTL2, a member of the EXT family of tumour suppressors, in liver injury and regeneration processes." in: The Biochemical journal, Vol. 454, Issue 1, pp. 133-45, 2013 (PubMed).