Goat anti-Rabbit IgG Antibody (DyLight 800) - Preadsorbed

Details for Product No. ABIN6699118
Antigen
Reactivity
Rabbit
355
342
336
233
232
122
113
87
80
73
72
65
61
57
54
49
43
31
27
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Goat
845
799
336
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Conjugate
DyLight 800
236
220
209
150
147
130
75
49
47
35
31
26
26
26
26
26
25
23
23
23
22
22
21
21
21
20
20
20
20
20
20
18
16
14
13
10
9
9
9
9
8
8
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Options
Immunogen

Immunogen: Rabbit IgG whole molecule

Isotype IgG
Characteristics

Synonyms: Goat anti-Rabbit IgG Antibody DyLight™800 Conjugation, Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody

Background: Anti-Rabbit IgG Antibody DyLight™800 generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to DyLight™800.

Purification Preadsorption: immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
Labeling Ratio 1.7
Antigen
Application Notes

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiry Date 12 months
Product cited in: Cao, Xue, Cheng, Wang, Liu, Li, Jiang, Li, Gui, Zhang: "MDM2 promotes genome instability by ubiquitinating the transcription factor HBP1." in: Oncogene, Vol. 38, Issue 24, pp. 4835-4855, 2019 (PubMed).

Heubl, Zhang, Pressey, Al Awabdh, Renner, Gomez-Castro, Moutkine, Eugène, Russeau, Kahle, Poncer, Lévi: "GABAA receptor dependent synaptic inhibition rapidly tunes KCC2 activity via the Cl--sensitive WNK1 kinase." in: Nature communications, Vol. 8, Issue 1, pp. 1776, 2018 (PubMed).

Xia, Ji, Xu, Lin, Wang, Xia, Lv, Song, Ma, Chen: "Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress." in: Cell death & disease, Vol. 8, Issue 7, pp. e2957, 2018 (PubMed).

Li, Xu, Lin, Qu, Xia, Hongdu, Xia, Wang, Lou, He, Ma, Chen: "Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality." in: Cell death & disease, Vol. 8, Issue 5, pp. e2811, 2018 (PubMed).

Lin, Cui, Xu, Hong, Xia, Xu, Li, Zhang, Lou, He, Lv, Chen: "Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy." in: Cell death & disease, Vol. 9, Issue 7, pp. 768, 2018 (PubMed).

Zhang, Kang, Zhou, Cui, Jia, Hu, Ji, Yuan, Cui, Shi: "Amelioratory Effects of Testosterone Propionate on Age-related Renal Fibrosis via Suppression of TGF-β1/Smad Signaling and Activation of Nrf2-ARE Signaling." in: Scientific reports, Vol. 8, Issue 1, pp. 10726, 2018 (PubMed).

Myles, Anderson, Earland, Zarember, Sastalla, Williams, Gough, Moore, Ganesan, Fowler, Laurence, Garofalo, Kuhns, Kieh, Saleem, Welch, Darnell, Gallin, Freeman, Holland, Datta: "TNF overproduction impairs epithelial staphylococcal response in hyper IgE syndrome." in: The Journal of clinical investigation, Vol. 128, Issue 8, pp. 3595-3604, 2018 (PubMed).

Wang, Cao, Xue, Li, Jiang, Cheng, Li, Zhang: "A positive feedback loop between Pim-1 kinase and HBP1 transcription factor contributes to hydrogen peroxide-induced premature senescence and apoptosis." in: The Journal of biological chemistry, Vol. 292, Issue 20, pp. 8207-8222, 2017 (PubMed).

Wang, Gregory-Evans, Wasan, Sivak, Shan, Gregory-Evans: "Efficacy of Postnatal In Vivo Nonsense Suppression Therapy in a Pax6 Mouse Model of Aniridia." in: Molecular therapy. Nucleic acids, Vol. 7, pp. 417-428, 2017 (PubMed).

Chen, Pan, Wang, Cao, Wang, Wang, Hu, Xue, Li, Jiang, Li, Zhang: "HBP1-mediated Regulation of p21 Protein through the Mdm2/p53 and TCF4/EZH2 Pathways and Its Impact on Cell Senescence and Tumorigenesis." in: The Journal of biological chemistry, Vol. 291, Issue 24, pp. 12688-705, 2016 (PubMed).

Shen, Kan, Hu, Li, Lu, Zhang, Zhang, Hou, Chen, Bai: "EMC6/TMEM93 suppresses glioblastoma proliferation by modulating autophagy." in: Cell death & disease, Vol. 7, pp. e2043, 2016 (PubMed).

Wang, Hu, Li, Qu, He, Lou, Song, Ma, Chen: "PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1." in: Autophagy, Vol. 10, Issue 12, pp. 2158-70, 2015 (PubMed).

Wang, Chen, Wang, Hu, Cao, Wang, Tong, Zhang: "PIASxα ligase enhances SUMO1 modification of PTEN protein as a SUMO E3 ligase." in: The Journal of biological chemistry, Vol. 289, Issue 6, pp. 3217-30, 2014 (PubMed).

Li, Zhao, Hu, Xiao, Qu, Wang, Ma, Chen: "A novel ER-localized transmembrane protein, EMC6, interacts with RAB5A and regulates cell autophagy." in: Autophagy, Vol. 9, Issue 2, pp. 150-63, 2013 (PubMed).