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Cytochrome C antibody (Cytochrome C, Somatic) (AA 1-80) Primary Antibody

CYCS Reactivity: Dog, Drosophila melanogaster, Frog, Horse, Human, Mouse, Pigeon, Rat FACS, IF, IHC, StM, WB Host: Mouse Monoclonal 7H8-2C12
Catalog No. ABIN6940319
$508.20
Plus shipping costs $45.00
100 μg ABIN6940319
100 μg ABIN6940319
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  • Target
    Binding Specificity
    AA 1-80
    Reactivity
    Dog, Drosophila melanogaster, Frog, Horse, Human, Mouse, Pigeon, Rat
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This Cytochrome C antibody is un-conjugated
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM), Western Blotting (WB)
    Specificity
    It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
    Purification
    Purified by Protein A/G
    Immunogen
    Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
    Clone
    7H8-2C12
    Isotype
    IgG2b, kappa
  • Application Notes

    Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

    Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Alternative Name
    CYCS (CYCS Antibody Abstract)
    Molecular Weight
    15kDa
    Gene ID
    54205
    UniProt
    P99999
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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